Caveolin3 Stabilizes McT1-Mediated Lactate/Proton Transport in Cardiomyocytes
CAV3 (caveolin3) variants associated with arrhythmogenic cardiomyopathy and muscular dystrophy can disrupt post-Golgi surface trafficking. As CAV1 (caveolin1) was recently identified in cardiomyocytes, we hypothesize that conserved isoform-specific protein/protein interactions orchestrate unique cardiomyocyte microdomain functions. To analyze the CAV1 versus CAV3 interactome, we employed unbiased live-cell proximity proteomic, isoform-specific affinity, and complexome profiling mass spectrometry techniques. We demonstrate the physiological relevance and loss-of-function mechanism of a novel CAV3 interactor in gene-edited human induced pluripotent stem cell cardiomyocytes.
To identify differential CAV1 versus CAV3 protein interactions and to define the molecular basis of cardiac CAV3 loss-of-function.
Methods and Results:
Combining stable isotope labeling with proximity proteomics, we applied mass spectrometry to screen for putative CAV3 interactors in living cardiomyocytes. Isoform-specific affinity proteomic and co-immunoprecipitation experiments confirmed the monocarboxylate transporter McT1 (monocarboxylate transporter type 1) versus aquaporin1, respectively, as CAV3 or CAV1 specific interactors in cardiomyocytes. Superresolution stimulated emission depletion microscopy showed distinct CAV1 versus CAV3 cluster distributions in cardiomyocyte transverse tubules. CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9 nuclease)-mediated CAV3 knockout uncovered a stabilizing role for McT1 surface expression, proton-coupled lactate shuttling, increased late Na+ currents, and early afterdepolarizations in human induced pluripotent stem cell-derived cardiomyocytes. Complexome profiling confirmed that McT1 and the Na,K-ATPase form labile protein assemblies with the multimeric CAV3 complex.
Combining the strengths of proximity and affinity proteomics, we identified isoform-specific CAV1 versus CAV3 binding partners in cardiomyocytes. McT1 represents a novel class of metabolically relevant CAV3-specific interactors close to mitochondria in cardiomyocyte transverse tubules. CAV3 knockout uncovered a previously unknown role for functional stabilization of McT1 in the surface membrane of human cardiomyocytes. Strikingly, CAV3 deficient cardiomyocytes exhibit action potential prolongation and instability, reproducing human reentry arrhythmias in silico. Given that lactate is a major substrate for stress adaption both in the healthy and the diseased human heart, future studies of conserved McT1/CAV3 interactions may provide rationales to target this muscle-specific assembly function therapeutically.
Editorial, see p 720
In This Issue, see p 687
Cardiomyocytes exhibit a high density of caveolae in the plasma membrane increasing their surface area up to 2-fold.1,2 Recent progress revealed how caveolae are molecularly stabilized through the coordinated actions of key lipid-binding proteins.1 The partly membrane-integrated caveolin family members CAV1 (caveolin1) and CAV3 (caveolin3), as well as Cavin1, were identified as essential cytosolic coat components that directly stabilize the characteristic omega-shaped caveolae structure in the surface membrane.1,3 Moreover, the caveolar neck constriction is stabilized through a ring-shaped protein complex, where EHD2 (Eps15 homology domain 2) inhibits caveolar endocytosis.3,4
Since CAV1 knockout mice develop heart failure and CAV1 is abundant in fibroblasts, it was assumed that cardiomyocytes are not primarily affected.5 In contrast, immuno-gold EM localized CAV1 in caveolae in human cardiomyocytes,6 and immunoblotting demonstrated CAV1 expression in isolated mouse cardiomyocytes.7 In addition, genome-wide studies have associated common CAV1 variants with cardiac conduction disease and atrial fibrillation.8 Accordingly, a cardiomyocyte-specific mechanism of conduction disease was demonstrated in CAV1 knockout hearts.7 However, neither the relationship between endogenous CAV1 and CAV3 nor human loss-of-function mechanisms were established in cardiomyocytes previously.
Both functionally important mechanoprotective and signal transduction roles have been assigned to caveolae.1 For example, in skeletal muscle, flattening and disassembly of caveolae in response to increased stretch protects membrane integrity during muscle contraction.2 In addition, G-protein coupled receptor and ion channel signaling have been associated with caveolae.2,9 However, the multitude of candidate protein interactors cumulatively assigned through heterologous overexpression studies contrasts with recent observations in gene-edited cell lines showing that bulk membrane proteins are relatively depleted in caveolae.10 Caveolar exclusion of membrane proteins was proposed to involve steric barriers provided by the coat and the ring protein complexes, as well as unfavorable membrane curvatures.3,10 However, neither were putative CAV3 nor CAV1 binding proteins functionally established in cardiomyocytes.1,2
CAV1 knockout mouse embryonic fibroblasts treated with dichloroacetate shift glucose utilization from lactate and pyruvate production to OXPHOS (mitochondrial oxidative phosphorylation electron transfer complexes) generation leading to cell death.11 This was linked to mitochondrial dysfunction due to abnormal cholesterol accumulation leading to impaired mitochondrial import of the key antioxidant glutathione, such that OXPHOS increases reactive oxygen species sufficiently to trigger apoptosis.11 Notably, an increase in apoptotic hepatocytes and neurons was confirmed in CAV1 knockout mice in vivo.11 Interestingly, in the failing human heart it has been shown that lactate is an important respiratory substrate, and lactate uptake through McT1 (monocarboxylate transporter type 1) is chronically increased, apparently compensating for decreased fatty acid utilization in mitochondrial energy production.12 Finally, during exercise lactate uptake is markedly increased in cardiomyocytes and mitochondrial lactate oxidation may account for over 50% of oxygen consumption in the human heart.12
In patients, rare CAV3 variants cause a hypertrophic cardiomyopathy classified as LQTS9 (long-QT syndrome type 9).9 Accordingly, overexpression of CAV3 containing the human F97C or S141R variants in HEK293 (human embryonic kidney cell 293) cells stably expressing the Na+ channel NaV1.5 resulted in increased late Na+ currents.9 On the contrary, CAV3 was found to co-purify with the dystrophin-glycoprotein complex presumably through its interaction with nitric oxide synthase,13 whereas NaV1.5 was shown to bind to the dystrophin-glycoprotein complex through α- and β-syntrophin.14 Hence, the molecular nature of putative protein/protein interactions between the homomeric CAV3 core complex, NaV1.5, the dystrophin-glycoprotein complex, and additional functional components remain unclear. As powerful live-cell proteomic and quantitative mass spectrometry techniques have emerged recently, we combined these to elucidate the spectrum of cardiac CAV1 and CAV3 protein interactions in an unbiased fashion.15,16
Here, we identify McT1 as a member of a new class of CAV3 binding proteins and a functional link to cardiac metabolism. While this interaction does not extend to the CAV1 isoform, McT1 and CAV3 occur in functionally important membrane domains, specifically in transverse (T-) tubules. In human induced pluripotent stem cell (iPSC)-cardiomyocytes CAV3 knockout uncovered a stabilizing role both for McT1 surface expression and co-transport of small monocarboxylates, particularly for lactate/proton shuttling. CAV3 knockout significantly prolonged repolarization, leading to early afterdepolarizations (EADs), increased late sodium currents (INa,L), and computational modeling confirmed the proarrhythmic effects. While reciprocal co-immunoprecipitations do not support a stable CAV3 interaction with NaV1.5 at endogenous levels in mature mouse cardiomyocytes, stimulated emission depletion (STED) superresolution imaging showed these proteins clustered in T-tubules in proximity to each other. Complexome profiling enhanced by in-cell cross-linking confirmed a functional proximity of McT1, the Na,K-ATPase, and the Na/Ca-exchanger with the CAV3 complex in cardiomyocytes. Hence, stabilization of functional McT1 expression in the surface membrane requires isoform specific CAV3 protein interactions to sustain mitochondrial substrate exchange and electrical membrane stability.
The authors declare that all supporting data including complete protein tables are available within the article and the Data Supplement. More detailed methods are provided in the Data Supplement. The data that support the findings of this study are available from the corresponding author upon reasonable request. Please see the Major Resources Table in the Data Supplement.
Ratiometric APEX2 Proximity Assay by 3-State SILAC-MS
We used the engineered peroxidase APEX2 (ascorbate peroxidase engineered X2) to genetically tag CAV3 in living cardiomyocytes to label endogenous proteins in nanometric proximity of the macromolecular CAV3 complex via biotinylation (Figure 1A). Bicistronic recombinant adenoviral vectors were generated to express V5-APEX2-CAV3 and enhanced grean fluorecent protein (eGFP) at the lowest effective multiplicity-of-infection in neonatal rat ventricular myocytes (NRCMs) for 48 hours in culture (Table I in the Data Supplement). In addition, adenoviral V5-APEX2 expression served as soluble control. For ratiometric proteomic analysis, we established 3-state stable isotope labeling by amino acids in cell culture (SILAC) labeling in NRCMs (Figure 2A and Table II in the Data Supplement). Based on ≥96.5% SILAC incorporation after 13 days (Figure 2B), adenoviral transfection occurred on day 11 for subsequent V5-APEX2-CAV3 or V5-APEX2 protein labeling. Following H202 treatment, differentially labeled cells were lysed and combined, and biotinylated proteins enriched by avidin. Avidin eluates were separated by SDS-PAGE into 12 fractions per lane, tryptically digested in-gel, and identified by data-dependent acquisition mass spectrometry using a Top12 method on a hybrid quadrupole/orbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific; Table III in the Data Supplement). Three independent SILAC cell cultures with label switches provided biological triplicates, each of which was analyzed by duplicate injections into the MS. Protein and peptide results were truncated to false discovery rates of 1%, respectively. MS1 ion chromatograms were extracted using MaxQuant 184.108.40.206 software (Max Planck Institute for Biochemistry) and used for ratiometric protein quantitation (Detailed Methods in the Data Supplement).
CAV1/CAV3 Interactome Analysis by AP-DIA-MS
For affinity purification-mass spectrometry, CAV1 and CAV3 were immunoprecipitated from 500 µg mouse ventricular tissues (Tables IV and V in the Data Supplement). Eluted proteins were purified as a single fraction using 4% to 12% gradient gels and tryptically digested in-gel. Rabbit IgG (12-370, Merck) was used as negative control. Digested samples were analyzed on a hybrid quadrupole/time-of-flight mass spectrometer (TripleTOF 5600+, SCIEX). For generation of a spectral library, a data-dependent acquisition mass spectrometry Top25 method was used and proteins identified using ProteinPilot 5.0 software (SCIEX). FDR was adjusted to 5% at this step. For quantitative analysis, data-independent acquisition mass spectrometry (DIA-MS) data were acquired using 65 variable size precursor windows across the 400 to 1050 m/z range. Three biological replicates per sample were analyzed by triplicate injection into the MS. MS2 ion chromatograms for 6 fragments per peptide and up to 10 peptides per protein were extracted from the data using PeakView 2.1 software (SCIEX) to a false detection rate of 1% and summed at the peptide and protein levels for statistical analysis (detailed methods in the Data Supplement).
BN-PAGE Analysis, Co-Immunoprecipitation, and Immunoblotting
Blue native polyacrylamide gel electrophoresis (BN-PAGE) was used to identify high molecular weight macromolecular complexes of the V5-APEX2-CAV3 fusion protein with endogenous CAV3 in NRCMs, as well as high molecular weight complexes of recombinant wild-type (WT) or endogenous mouse CAV3 (Tables VI and VII in the Data Supplement). Reciprocal co-immunoprecipitations followed by immunoblotting were used to confirm isoform specific CAV1 versus CAV3 protein interactions.
Complexome Profiling by BN-PAGE-MS
Mouse ventricular cardiomyocytes were subjected to in-cell protein cross-linking using the homobifunctional cross-linker disuccinimidyl-suberate (DSS 0.1 mol/L, 1 hour) to stabilize labile protein/protein complexes. Following lysis, proteins and protein/protein complexes were separated by BN-PAGE, visualized by Coomassie staining and cut into 23 equidistant gel fractions. Proteins were tryptically digested in-gel and analyzed by data-dependent acquisition mass spectrometry using a Top-15 method on a hybrid quadrupole/orbitrap mass spectrometer (Q Exactive Plus, Thermo Fisher Scientific). Three biological replicates per sample were analyzed by duplicate injections into the MS. Protein and peptide results were truncated to false discovery rates of 5%, respectively, to generate comprehensive candidate lists for hypothesis-driven data exploration. MS1 ion chromatograms were extracted using MaxQuant 220.127.116.11 software (Max Planck Institute for Biochemistry), combined to protein abundance values, normalized, and visualized as heat maps and line profiles (detailed methods in the Data Supplement).
Human CAV3 Knockout iPSC-Cardiomyocytes
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9 nuclease)-mediated genome editing in human iPSCs was applied to generate CAV3 knockout lines, and engineered iPSCs were directly differentiated into ventricular-like cardiomyocytes for functional analysis (Tables IX through XI in the Data Supplement). The study was approved by the Ethics Committee (approval number 10/9/15) and performed in accordance with approved guidelines.
Computational Modeling of Human iPSC-Cardiomyocytes
Single-cell and tissue-level simulations were performed using the original Kernik human iPSC-cardiomyocyte model,17 as well as a version prone to EAD development in the presence or absence of experimentally observed ion channel changes associated with CAV3 knockout (detailed methods and Table XIII in the Data Supplement).
Data are presented as mean±SEM unless indicated otherwise. Unpaired 2-tailed Student t test or 1-way ANOVA was applied as specified in the figure legends. A P value <0.05 was considered as statistically significant (detailed methods in the Data Supplement).
Targeting the Macromolecular CAV3 Complex for Live-Cell Proteomics
To label endogenous proteins in cardiomyocytes, we developed an N-terminally tagged V5-APEX2-CAV3 tool construct (Figure 1A). APEX2, an engineered peroxidase, is used to biotinylate proteins in nanometric proximity (ie, ≤20 nm radius) to CAV3 in living cells upon H2O2 treatment for identification by affinity purification and mass spectrometric analysis of labeled proteins (detailed methods in the Data Supplement).15,16 We hypothesized that V5-APEX2-CAV3 and endogenous CAV3 form a multimeric protein complex in NRCMs if their expression levels are similar. Using adenoviral vectors, we titrated the multiplicity of infection down to the lowest effective dose (multiplicity-of-infection) and immunoblotting confirmed V5-APEX2-CAV3 expression levels similar to endogenous CAV3 (Figure 1B). Since plasmid-transfected NRCMs showed a significantly lower V5-APEX2-CAV3 expression (Figure 1B), we used adenoviral transfection of V5-APEX2-CAV3 (multiplicity-of-infection) henceforth. As CAV3 expression in Sf9 cells produced a stable disc-shaped nonameric complex,18 we asked if V5-APEX2-CAV3 is competent to bind endogenous CAV3 in a heteromeric complex?
First, we used co-immunoprecipitation followed by V5 and CAV3 immunoblotting to exclude unspecific interactions with the soluble V5-APEX2 control. Supporting our hypothesis, co-immunoprecipitation of V5-APEX2-CAV3 confirmed endogenous CAV3 as binding partner (Figure 1C). Second, BN gradient gel analysis showed a high molecular weight complex under nondenaturating conditions. CAV3 immunoblotting confirmed a major complex at ≈545 kDa both in untransfected and V5-APEX2-CAV3 transfected NRCMs (Figure 1D). In addition, V5 immunoblotting identified V5-APEX2-CAV3 unambiguously as exogenous component of the ≈545 kDa complex (Figure 1D). To assess protein biotinylation in NRCMs qualitatively, we used avidin-affinity purification (detailed methods in the Data Supplement). This confirmed that V5-APEX2-CAV3 robustly labels endogenous proteins through biotinylation in living NRCMs treated for 1 minute with H2O2 (Figure IA and IB in the Data Supplement). Finally, confocal imaging confirmed that V5-APEX2-CAV3 co-localizes with endogenous CAV3 in NRCMs (Figure 1E; for muliplicity of infections [MOIs] >1 see Figure IC in the Data Supplement).
Quantitative CAV3 Proximity Proteomics in Cardiomyocytes
To develop a quantitative proteomic approach, we established a 3-state SILAC workflow for systematic label switching (Figure 2A and Figure IIA in the Data Supplement). NRCMs cultured in SILAC media expressing V5-APEX2-CAV3 showed ≥96.5% isotope label incorporation (Figure 2B). As ratiometric controls, we used adenoviral transfections of V5-APEX2 and eGFP based on published protocols.16,19 V5-APEX2-CAV3 and V5-APEX2 expression was confirmed by V5 immunoblotting (Figure 2C). Biotinylated proteins were enriched by affinity purification and affinity purification-mass spectrometry identified 1131 biotinylated proteins, of which 101 proteins including 9 proteins of interest were significantly enriched for V5-APEX2-CAV3 (Figure 2D and data set Excel Table I in the Data Supplement). As expected for CAV3 protein complexes assembled in the endoplasmic reticulum (ER) and Golgi followed by vesicular trafficking to the surface membrane, V5-APEX2-CAV3 labeling occurred in the proximity of different organelles, for example in ER-associated mitochondria (Figure IIB in the Data Supplement).11 Electron tomography confirmed that caveolae are situated in <10 nm proximity to mitochondria in cardiomyocytes (Figure IIC through IIE and Table VIII in the Data Supplement), and, thus, within the expected 20 nm radius of the reactive V5-APEX2-CAV3 generated biotin labeling cloud.15,16
Using the STRING (Search Tool for Retrieval of Interacting Genes/Proteins) protein/protein interaction database,20 we mapped the interaction networks of the identified proteins of interests using the GO (GO term functional classification framework) terms caveolae, muscle contraction, pyruvate metabolism, and iron uptake and transport (Figure 2E). Confirming our proximity proteomic strategy, all essential and muscle-specific components of the core caveolar complex, namely CAV3, Cavin1, and Cavin4 were identified consistent with earlier studies.21,22 Surprisingly, we identified CAV1, while Pacsin2 has been associated with caveolae previously.23 Moreover, Myl (myosin light chain; Myl2, Myl3, and Myl6), Act (actin; Acta1, Acta2, and Actc1), and Tnni1 (troponin) were identified together with the Na,K-ATPase α1 and β1 subunits, and the Ncx1 (Na/Ca exchanger) confirming earlier studies.24,25 Finally, proteins that define key transmembrane metabolic substrate carriers were detected (data set Excel Table I in the Data Supplement). Importantly, we identified the monocarboxylate transporter McT1 and the transferrin receptor TfR1 (transferrin receptor 1) as new CAV3 proximity candidates (Figure 2E).
Differential CAV1 Versus CAV3 Expression in Cardiomyocytes
As the presence of CAV1 in striated muscle cells remains controversial,5,26 we validated CAV1 protein expression in lysates of ventricular cardiomyocytes isolated from adult mouse hearts. Immunoblotting confirmed that CAV1 is robustly expressed, visible as a single band below 25 kDa, while antibody specificity was confirmed in CAV1 knockout mouse hearts (Figure 3A and Figure IIIA in the Data Supplement). Moreover, label-free DIA-MS established the expression of all 3 mammalian CAV isoforms in isolated cardiomyocytes, surprisingly with the highest protein area measured for CAV1 (Figure 3B and data set Excel Tables II and III in the Data Supplement). DIA-MS protein areas were previously demonstrated to correlate strongly with absolute cellular protein concentrations even without the use of quantified standards.27 Out of 1816 proteins detected, the ubiquitous CAV1 isoform was ranked No. 205 by protein area and thus highly abundant, whereas the muscle-specific CAV3 ranked No. 1105 indicating a much lower abundance (Figure 3C).
To investigate the relationship between CAV1 and CAV3 in adult ventricular cardiomyocytes, we used confocal and STED superresolution microscopy (nanoscopy). STED nanoscopy resolved CAV1 cluster signals in physiologically relevant membrane structures, namely the intercalated disk and T-tubules, but not in the lateral surface membrane (Figure 3D). While the CAV1 and CAV3 signals occurred frequently adjacent to each other in T-tubules, STED did not resolve co-localized signals (Figure 3D). Unspecific CAV1 signals in T-tubules were excluded using murine CAV1 knockout cardiomyocytes as negative control (Figure IIIB in the Data Supplement). Finally, reciprocal immunoprecipitation of CAV1 versus CAV3 in cardiomyocyte lysates indicated relatively weak heteromeric versus strong homomeric protein interactions (Figure 3E). Hence, CAV1 and CAV3 clusters are differentially distributed in cardiomyocytes, presumably each in isoform-specific homomeric core complexes, but frequently positioned near each other in T-tubule nanostructures.
Isoform-Specific CAV Interactions
To explore the hypothesis that CAV1 and CAV3 provide macromolecular scaffolds for differential subcellular protein interactions, we analyzed mouse ventricular tissue lysates by immunoprecipitation followed by quantitative DIA-MS. CAV1 and CAV3 were readily differentiated by the detection and quantification of multiple isoform-specific peptides following tryptic digestion of immunoprecipitation eluates (Figure IVA and IVB and data set Excel Tables II and III in the Data Supplement). We detected 62 potential protein interactions for CAV1 (Figure VA in the Data Supplement) and 70 for CAV3 (Figure VB in the Data Supplement) using permutation-based false discovery rate analysis (P<0.05).28 To identify isoform-specific interactions, we compared the respective CAV1/CAV3 enrichment ratios and found 7 interactors specific for CAV1 versus 23 for CAV3 (Figure 4A). Importantly, among proteins of interests with an established pathophysiological role, affinity-based DIA-MS confirmed McT1 as a previously unknown CAV3 interactor (Figure 4A). Next, we compared the fold change of the logarithmic ratio each for CAV1 or CAV3 normalized to IgG (Figure 4B), confirming preferential CAV1 interactions with aquaporin-1, CAV1, CAV2, Cavin1, and Cavin2. In contrast, CAV3 binds preferentially to itself, the insulin-dependent glucose transporter GluT4, to McT1, the Na,K-ATPase α1 and β1 subunits, Connexin43, and Ncx1. Consistent with the concept of preferential homomeric isoform-specific complexes, significant heteromeric interactions between CAV1 and CAV3 were not detected by affinity-based DIA-MS (Figure 4B).
To validate our findings, we performed reciprocal immunoprecipitation experiments followed by immunoblotting in mouse ventricular tissue lysates. Whereas CAV1 showed an exclusive interaction with aquaporin1, we confirmed isoform specific CAV3 interactions with connexin43, McT1, and TfR1. Again, relatively weak heteromeric interactions between CAV1 and CAV3 were evidenced, contrasting with strong homomeric self-interactions (Figure 4C). Finally, STED nanoscopy resolved McT1 clusters as punctate signals both in the lateral surface membrane and in T-tubules of adult mouse cardiomyocytes, and consistent with differential subcellular CAV3 versus CAV1 distributions (Figure 4D). As small McT1 clusters were frequently localized on the edge or inside CAV3 clusters, we propose that McT1 is functionally associated with CAV3 complexes in the plasma membrane. Taken together, these data confirm isoform-specific protein interactions of CAV3 with McT1 and highlight their physiologically relevant location in T-tubules in nanometric proximity to mitochondria (Figure IID and IIE in the Data Supplement).
CAV3 Knockout Affects McT1 Surface Expression in Human Cardiomyocytes
We hypothesized that protein/protein interactions with the CAV3 complex stabilize functional McT1 expression in the surface membrane. To test this, we generated a human iPSC knockout model (CAV3 knockout) targeting the start codon of exon 1 by CRISPR/Cas9 (Figure VI in the Data Supplement). Immunoblotting of ventricular-like cardiomyocyte lysates derived from WT iPSC confirmed robust expression of CAV3 and McT1 (Figure 5A). In contrast, CAV3 knockout iPSC-cardiomyocytes (iPSC-CMs) were completely CAV3 deficient, whereas McT1 protein expression was decreased (Figure 5A). To explore if the McT1 decrease is functionally relevant in the sarcolemma, we applied extracellular surface biotinylation to living cardiomyocytes in culture. Surface biotinylated proteins were enriched by pull-down and McT1 identified by immunoblotting. Indeed, McT1 was specifically decreased in the surface membrane of CAV3 knockout cardiomyocytes (Figure 5B).
To investigate if decreased McT1 surface expression functionally impacts substrate uptake, we exposed human iPSC-CMs to extracellular 3-bromopyruvate, a glycolysis-disrupting compound previously established as McT1-specific substrate.29,30 Accordingly, WT and CAV3 knockout cardiomyocytes were treated with 3-bromopyruvate (50 µmol/L), and cell viability was assessed by LDH (lactate dehydrogenase) release using published protocols.31 Consistent with McT1 loss in the surface membrane leading to decreased 3-bromopyruvate uptake, LDH release was significantly decreased in human CAV3 knockout iPSC-CMs (Figure 5C).
McT1 is thought to represent the major pathway for transmembrane lactate and pyruvate transport in the heart32 and is upregulated in heart failure.12 To assess if human CAV3 knockout cardiomyocytes experience substrate-dependent metabolic limitations, we measured oxygen consumption and extracellular acidification using Seahorse protocols established for iPSC-CMs previously (Table XI in the Data Supplement).33 While oxygen consumption was normal in CAV3 knockout cardiomyocytes (Figure 5D), extracellular acidification was significantly decreased (Figure 5E). Moreover, inhibiting ATP synthesis with oligomycin increased extracellular acidification maximally in WT but not in CAV3 knockout cardiomyocytes consistent with impaired proton-coupled lactate export (Figure 5E). Finally, mitochondrial uncoupling by ionophore (FCCP [carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone]) or electron transport chain inhibition (antimycin+rotenone) did not result in further changes. Together, these experiments established that CAV3 knockout destabilizes functional McT1 surface expression and extracellular acidification through the lactate/proton shuttle in human iPSC-CMs.
CAV3 Deficiency Increases APD and Promotes EADs
Based on optical recording of spontaneous iPSC-CM depolarizations using the voltage-sensitive dye FluoVolt (final kit dilution 0.1× according to manufacturer instructions; detailed methods in the Data Supplement), action potentials from CAV3 knockout compared with WT cells showed a profound delay of repolarization (Figure 6A). Both individual and averaged action potential duration (APD) values at 50% and 90% of repolarization (APD50 and APD90) were significantly prolonged in CAV3 knockout iPSC-CMs (Figure 6B).
In addition to APD prolongation, the relationship between early and late repolarization given by the repolarization fraction ([APD90−APD50]/APD90) was significantly increased in CAV3 knockout iPSC-CMs (Figure 6C). This indicates that CAV3 knockout may not only delay repolarization but promote beat-to-beat electrical instability. Indeed, spontaneously generated action potentials were followed by singular as well as repetitive (regenerative) APDs (Figure 6D). On average, in the absence of any pharmacological interventions 35% of the human CAV3 knockout cells produced EADs in sharp contrast to 0% of the WT control iPSC-CMs (Figure 6E). As human CAV3 variants associated with LQTS9 were shown to increase late Na+ currents after overexpression in HEK293 cells,9 we aimed to confirm the putative NaV1.5 channel interaction under physiological conditions in iPSC-CMs.
Whole-cell patch-clamp measurements showed similar Na+ current (INa) behaviors in WT and CAV3 knockout iPSC-CMs throughout step-wise depolarizations (Figure 6F, left). Comparing current density-voltage relations between WT versus CAV3 knockout showed no difference, particularly no significant change of the peak INa (Figure 6F, right). However, an increased TTX (tetrodotoxin)-sensitive INa,L was observed in CAV3 knockout iPSC-CMs (Figure 6G, left). Accordingly, the calculated INa,L integral confirmed a significant INa,L increase in human CAV3 knockout iPSC-CMs (Figure 6G, right).
Proximity proteomics has identified both Na,K-ATPase subunits near CAV3 (Figure 2). However, direct Na,K-ATPase interactions through a binding motif, essential for organogenesis, were recently established only for CAV1.34 Patch-clamp recording in WT versus CAV3 knockout iPSC-CMs showed similar activation and inhibition of Na,K-ATPase currents following 5 mmol/L extracellular K+ or 10 µmol/L ouabain, respectively (Figure VIIA and VIIB and Table XII in the Data Supplement). Accordingly, activation followed by inhibition of the Na,K-ATPase pump did not reveal any significant current differences in CAV3 knockout iPSC-CMs (Figure VIIC in the Data Supplement). Taken together, CAV3 knockout leads to decreased lactate/proton extrusion, increased late INa,L currents, and APD prolongation with EAD instability in iPSC-CMs.
We employed computational modeling to investigate the specific contribution of INa,L to EAD generation and tissue-level proarrhythmic consequences. At baseline, the WT iPSC-CM model does not have an appreciable INa,L consistent with our WT experimental data, while incorporating the increased INa,L associated with CAV3 knockout (Figure VIII and Table XIII in the Data Supplement) produced a minor APD prolongation (Figure 6H, EAD-resistant cell). However, models prone to EAD development due to additional changes in ICa,L and reduced repolarization reserve, based on experimental data,35,36 had a significantly longer baseline APD and developed runs of EADs in the presence of INa,L upregulation (Figure 6H, EAD-prone cell), thus resembling the electrophysiological phenotype observed in CAV3 knockout iPSC-CMs (Figure 6A through 6E).
Incorporating a mix of EAD-resistant and EAD-prone cells in 2-dimensional tissue simulations revealed their proarrhythmic potential. In the presence of a sufficiently large area of EAD-prone cells, tissue simulations exhibited (repetitive) focal activity (Figure IX and Movie I in the Data Supplement). Moreover, under specific conditions (involving the location of EAD-prone cells relative to the normal activation wave front and the APD of EAD-prone cells), EAD-prone cells can also elicit a figure-of-eight reentry pattern (Figure 6I, Figure X and Movie II in the Data Supplement), confirming the proarrhythmic phenotype observed in CAV3 knockout cells at the tissue level in silico.
CAV3 Does Not Co-Immunoprecipitate or Co-Localize With NaV1.5 Channels
Using a CAV-rich rat cardiomyocyte membrane fraction for CAV3 immunoprecipitation, immunoblotting suggested the cardiac Na+ channel as associated protein.37 When WT CAV3 was overexpressed with human NaV1.5 in HEK293 cells, immunoblotting after CAV3 immunoprecipitation indicated a similar protein association.9 However, the reciprocal NaV1.5 immunoprecipitation confirming CAV3 as putative interaction partner was not established.
First, using the protocol of Vatta et al,9 CAV3 versus CAV1 were immunoprecipitated in WT mouse ventricular lysates followed by NaV1.5 immunoblotting. While the Na+ channel was confirmed in the input fraction, NaV1.5 was not detected after immunoprecipitation (Figure XIA in the Data Supplement), while strong isoform-specific CAV1 and CAV3 self-interactions were confirmed (Figure XIB in the Data Supplement).
Second, we used a cardiac-restricted double-transgenic model expressing human FLAG-NaV1.5 to immunoprecipitate the protein via FLAG-antibody in mouse heart lysates.38 Immunoblotting confirmed human FLAG-NaV1.5, mouse NaV1.5, and CAV3 in the input fraction (Figure 7A). Following anti-FLAG immunoprecipitation, immunoblotting confirmed both the FLAG and human NaV1.5, whereas mouse NaV1.5 was not detected (Figure 7A). While CAV3 was abundantly present (input), however, CAV3 was not detected after human FLAG-NaV1.5 immunoprecipitation (Figure 7A).
Third, using a protocol that supports co-immunoprecipitation of the PDZ (PDZ domain) domain-binding protein SAP97 together with NaV1.5 by Milstein et al,39 we tested the putative CAV3 interaction in WT mouse ventricular lysates. Whereas immunoblotting identified NaV1.5 and SAP97 (positive control) after immunoprecipitation, rabbit IgG served as negative control. However, while CAV3 was present (input), only the direct NaV1.5 interactor SAP97 but not CAV3 were detected (Figure 7B).
Finally, since co-localization of CAV3 and NaV1.5 both in human and rat hearts has been reported previously,9,37 we performed high-resolution confocal and STED imaging studies in mouse cardiomyocytes. Interestingly, confocal imaging frequently showed yellow signal spots in transversal striations suggesting that CAV3 and NaV1.5 may be co-localized in T-tubules (Figure 7C). In contrast, by switching on the STED laser beam, CAV3 versus NaV1.5 signals were resolved as spatially separate clusters in T-tubule striations but apparently not co-localized (Figure 7C). Hence, neither co-immunoprecipitation nor co-localization, consistent with proximity- and affinity-based MS, can support the previously proposed interaction between NaV1.5 and CAV3 at physiological protein levels in mature mouse cardiomyocytes.
Complexome Profiling Confirms Labile Protein Assemblies With the CAV3 Complex
To resolve the association of multimeric CAV3 complexes with McT1 and cardiac ion transport complexes according to apparent weight, we used BN-PAGE separation followed by mass spectrometry (BN-PAGE-MS). Untreated versus DSS treated (0.1 mmol/L, 1 hour) mouse ventricular cardiomyocytes were analyzed to test if in-cell cross-linking enhances the detection of labile protein/protein complexes (methods and detailed methods in the Data Supplement). Protein complexes of membrane fractions each from untreated and DSS treated cells were separated on BN-PAGE gradient gels (Figure XIIA in the Data Supplement).
Each BN-PAGE lane was cut into 23 equally sized slices for in-gel tryptic digestion and data-dependent acquisition mass spectrometry. For mass calibration, we used a standard set of membrane proteins and protein complexes following protocols established previously (Figure XIIB in the Data Supplement).40 Two thousand four hundred sixty proteins were reproducibly detected in both conditions (Figure XIIC and data set Excel Table IV in the Data Supplement). Normalized BN-PAGE-MS intensity mapping identified the CAV3 complex at an apparent weight of ≥300 kDa in untreated cardiomyocytes (Figure 8A). Interestingly, McT1, the Na,K-ATPase α1/β1, Ncx1, and TfR1 were detected nearby at 100 to 300 kDa (Figure 8A). Abundance line profiling confirmed that the CAV3 complex abundance peak was clearly separated in mass from the McT1, Na,K-ATPase α1, and Ncx1 peaks with minimal overlap in the 100 to 400 kDa range (Figure 8B).
While the abundance pattern was similar for the CAV3 complex following live-cell DSS cross-linking (0.1 mmol/L), apparently McT1, Na,K-ATPase α1/β1, Ncx1, and TfR1 were shifted leftward towards the 100 to 400 kDa range (Figure 8C). Line profile plots confirmed that DSS cross-linking resulted in left-shifted major abundance fractions and additional minor peaks each for McT1, Na,K-ATPase α1, and Ncx1, overlapping with the CAV3 complex (Figure 8D). As DSS cross-linking captures proteins in direct proximity due to the restricted length of the cross-linker, McT1, the Na,K-ATPase, and Ncx1 may form labile protein assemblies with the CAV3 isoform complex, stabilizing electro-metabolic transport functions in a highly localized fashion in cholesterol-rich microdomains in cardiomyocytes.
Combining the strengths of proximity- and affinity-based proteomics, we have identified previously unknown, isoform-specific CAV1 versus CAV3 protein interactions in cardiomyocytes. Reciprocal co-immunoprecipitation experiments confirmed McT1 and TfR1 as new CAV3-specific binding partners of immediate relevance for cardiac metabolism and aquaporin1 as a CAV1-specific interactor. Surprisingly, quantitative proteomics identified CAV1 as a highly abundant isoform in cardiomyocytes relative to less abundant muscle-specific CAV3. Consistent with a functionally important and CAV3-dependent McT1 interaction in the surface membrane, CAV3 knockout uncovered a stabilizing role for transmembrane lactate/proton shuttling in human cardiomyocytes. Moreover, CAV3 knockout is sufficient to significantly increase INa,L leading to delayed AP repolarization and EADs in iPSC-CMs, and arrhythmias in mathematical iPSC-CM and tissue models, reminiscent of the human CAV3 variant LQTS9 phenotype in patients. At endogenous CAV3 and NaV1.5 protein levels in mouse hearts a functional association in T-tubules may occur through nanometric cluster proximities, while 2 independent co-immunoprecipitation approaches exclude stable protein interactions. Importantly, while we established the first physiological human iPSC-CM CAV3 knockout model exhibiting McT1 surface destabilization and electro-metabolic dysfunction, complexome profiling confirmed labile protein assemblies of McT1, the Na,K-ATPase, and Ncx1 with the CAV3 complex in living adult mouse cardiomyocytes. While a constitutive McT1 surface expression in cardiomyocytes was proposed previously,41 our data define the CAV3 complex as a muscle-specific protein interaction hub with major implications for cardiac cell biology, local electro-metabolic transmembrane transporter organization, and specific CAV3 loss-of-function phenotypes.
STED nanoscopy showed that CAV3 and McT1 are frequently co-clustered in the lateral cardiomyocyte surface membrane and in T-tubules. Immunogold EM studies demonstrated previously that McT1 is highly abundant in caveolae, the intercalated disks, and T-tubules, but only the latter showed dense immunogold labeling in direct proximity to mitochondria.41 While this suggests a privileged McT1-mitochondrial metabolite shuttling pathway at T-tubules, we identified CAV3 as an isoform-specific interactor of McT1. CRISPR/Cas9 knockout in human cardiomyocytes established a causal functional role, since McT1 expression was specifically decreased in the plasma membrane of CAV3 deficient cells. McT1 is known to facilitate the proton-coupled transport of small monocarboxylates, most importantly of lactate and pyruvate.32 During exercise lactate represents a major cardiac energy source that may account for over 50% of oxygen consumption.42 Whereas cardiac ischemia drives lactate efflux from affected cells,43 chronic heart failure leads to significantly increased McT1 protein expression, and lactate uptake.12
Given that McT1 has a prominent role in cardiac stress adaptation, our discovery that CAV3 interacts with McT1, functionally stabilizes substrate metabolism, and electrical membrane stability in human cardiomyocytes is highly relevant for the healthy and diseased heart. As cultured iPSC-derived cardiomyocytes predominantly use glucose for ATP production,44 energy homeostasis depends directly on McT1 surface expression and proton-coupled metabolite export.32 Consistent with this model CAV3 knockout resulted in significantly decreased extracellular acidification. Interestingly, pharmacological inhibition of monocarboxylate transport in tumor cells rapidly increases intracellular lactate, whereas ATP and glutathione synthesis are decreased, contributing to mitochondrial dysfunction.45
Quantitative proteomics and superresolution imaging established that CAV1 is highly abundant in the intercalated disks and T-tubules in adult mouse cardiomyocytes. Our data thus overcomes the prevailing notion that CAV1 is expressed predominantly in nonmuscle cells.1 In addition, immunolabeling freeze-fracture EM studies localized CAV1 in caveolae of human heart sections previously.6 Here, STED nanoscopy showed that CAV1 and CAV3 are not co-localized, although clusters of the 2 isoforms occurred frequently adjacent to each other in T-tubules. This supports the new hypothesis of isoform-specific subcellular nanodomain functions based on unique protein interactions of CAV1 versus CAV3 complexes. While an in-depth analysis of the CAV1 complex and confirmation of aquaporin 1 as a newly identified candidate interactor remain important questions for future studies, our findings offer a new cell biological perspective. CAV3 does not function as predominant but rather as additional muscle-specific isoform to locally stabilize and augment a unique set of essential metabolic functions (ie, McT1, Ncx1, Na,K-ATPase transport in T-tubules in proximity to mitochondria). Vice versa, the historic notion that CAV1 has no role in cardiomyocytes needs careful reinvestigation. As CAV1 is highly abundant in mature mouse cardiomyocytes, it seems unlikely that CAV1 knockout mice develop heart failure mainly from fibroblast defects.5 Furthermore, our findings have important implications for patients with CAV1 or CAV3 variants.
In line with our proteomic data (Figure 4A and 4B), the CAV3 variant P104L associated with limb girdle muscular dystrophy was shown to diminish insulin-induced surface expression of GluT4 and glucose uptake in skeletal myotubes.46 Interestingly, Golgi accumulation and mechanosensing defects were recently demonstrated for the CAV3 variants P28L and R26Q in skeletal myofibers of patient biopsies.47 This raises the possibility that pathogenic CAV3 variants affect both the heart and skeletal muscles through multi-factorial defects including (1) Golgi accumulation and protein-lipid aggregation; (2) disrupted surface trafficking and (3) cholesterol surface transport leading to mitochondrial toxicity11; (4) local metabolic substrate transport via Glut446 and McT141; (5) local electrophysiologically important ion transport interactions with Nav1.5 (Figures 6A through 6I and 7C), and putatively with Ncx125; (6) mechano-protective membrane plasticity2; and (7) mechanosensing.47 Additionally, CAV3 may interact with the AT1R (angiotensin II receptor) during exocytic transport, whereas the human CAV3-P104L variant disrupts AT1R surface trafficking.48 Moreover, CAV3 knockout in mice induces hypertrophy and cardiomyopathy, whereas cardiomyocyte-restricted CAV3 overexpression attenuated cardiac hypertrophy.49 Finally, while the CAV1 variant P132L was associated with extracardiac pathologies, CAV1-P132L overexpression resulted in Golgi accumulation.50 Taken together, our findings of isoform-specific protein interactions provide a template for future studies to determine pathogenic CAV3 mechanisms as well as the currently unknown impact of a large spectrum of human CAV1 variants in the context of cardiac and skeletal muscle dysfunction.
CAV1 knockout in mice has been shown to decrease left-ventricular conduction velocity through decreased connexin43 expression.7 As STED imaging showed few residual CAV1 signals in CAV1 knockout mouse cardiomyocytes at the intercalated disc but not in T-tubules, we have to assume some unspecific local antibody binding. Nonetheless, we confirmed robust CAV1 expression in mouse cardiomyocytes by immunoblotting in WT versus CAV1 knockout mouse cardiomyocytes and quantitatively by DIA-MS in mouse ventricles. Importantly, our proteomic analysis showed isoform–specific CAV3 interactions with connexin43 and Ncx1, whereas aquaporin 1 was confirmed as a CAV1-specific interactor through co-immunoprecipitation experiments. While human CAV1 and CAV3 are 61% identical, only CAV1 exhibits an extended N-terminal domain subject to Src (nonreceptor protein tyrosine kinase) phosphorylation at tyrosine-14, augmenting Src binding to CAV1.51 As the CAV1 variant P132L represents a well-established model of disrupted caveolae biogenesis,50 follow-up studies will need to explore the impact of this particular human variant and putative aquaporin 1 interactions in cardiomyocytes.
Excessive overexpression in heterologous cell systems has been shown to interfere with caveolae biogenesis, to lead to aberrant CAV1 trafficking, and to increase the pool of ectopic noncaveolar CAV1.50,52 For example, 4 hours after CAV1 transfection in fibroblasts most of the overexpressed CAV1 failed to co-localize with endogenous CAV1 in caveolae, instead accumulating in the late endosome.53 Notably, overexpression of V5-APEX2-CAV3 at an muliplicity of infection (MOI) ≥3 resulted in Golgi aggregates in NRCMs. Therefore, we have carefully titrated adenoviral expression of V5-APEX2-CAV3 to the lowest effective dose, demonstrating a similar distribution as endogenous CAV3 indicative of preserved CAV3 surface trafficking. Consistent with proteomic toxicity and our observations, 3-fold transgenic overexpression of WT CAV3 causes a degenerative cardiomyopathy in mice with severely diminished sarcolemmal dystrophin expression.54
Genome editing of NIH3T3 cells enabled CAV1 expression at low endogenous levels, demonstrating recently that caveolae are endocytosed at a very low rate, while bulk membrane proteins are effectively excluded from caveolae.10 In contrast, earlier studies used quantitatively uncontrolled overexpression of LQTS9 associated CAV3 variants in HEK293 cells to infer ectopic interactions with human NaV1.5 increasing INa,L currents.9 Affinity- and proximity-based proteomic analysis did not detect NaV1.5 as putative CAV1 or CAV3 interactor in rodent cardiomyocytes. However, human CAV3 knockout iPSC-CMs revealed ≈2-fold increased INa,L contributing to EAD instability, a directly proarrhythmogenic mechanism confirmed in mathematical iPSC-CM and tissue modeling by focal and reentry-type arrhythmias. Two independent, reciprocal CAV3 versus NaV1.5 co-immunoprecipitation approaches failed (based on the same protocols published previously9,39) to confirm the ectopic interaction observed in HEK293 cells possibly due to the lower physiological protein levels. However, STED nanoscopy showed that CAV3 and NaV1.5 clusters exist frequently in nanometric proximity to each other in T-tubules of mouse cardiomyocytes, supporting a local functional interaction, without co-localization as reported previously by light diffraction-limited confocal imaging.9 Hence, our observation that CAV3 knockout destabilizes lactate/proton shuttling and causes electrical instability in human iPSC-CMs introduces a novel loss-of-function and a unifying disease model relevant for follow-up studies of trafficking-incompetent CAV1 and CAV3 variants.
The proximity proteomic analysis used immature NRCMs and CAV3 knockout occurred in immature human iPSC-CMs, which may not translate to the adult situation. Therefore, we applied an in-depth multiplexed strategy followed by immunoprecipitation affinity purification-mass spectrometry and co-immunoprecipitation experiments to confirm or reject candidate CAV3 interactors in adult mouse heart lysates. Data from the ratiometric APEX2 proximity assay were searched against Rattus norvegicus UniProtKB database. While it is not complete with regard to cardiac proteins, we consciously chose not to use a combined murine database to avoid ambiguities by protein entry mapping. In addition, complexome profiling confirmed labile protein assemblies in adult mouse cardiomyocytes. Finally, subcellular in situ analysis of McT1, NaV1.5, CAV1, and CAV3 cluster locations by confocal and STED imaging was performed in adult mouse cardiomyocytes, and nanodomain proximities of caveolae at the surface and intracellular T-tubules with mitochondria were confirmed by electron tomography.
For a majority of proteins, information regarding their participation in functionally important macromolecular complexes is still lacking. For this purpose, we employed an optimized approach for complexome profiling comprising BN-PAGE separation and mass spectrometry, enhanced by in-cell cross-linking to stabilize labile interactions otherwise lost during analysis. This confirmed a labile protein assembly of McT1, Ncx1, and the Na,K-ATPase with the CAV3 complex in cardiomyocytes. In summary, we applied APEX2 proximity proteomics identifying McT1 as putative CAV3 interactor in NRCMs. As a second isoform-specific approach, affinity purification-mass spectrometry was used to establish CAV1-versus CAV3-specific interactors. For CAV3, we identified McT1 and TfR1 as novel isoform-specific interactors relevant for cardiac energy metabolism, whereas aquaporin 1 was identified for CAV1. Hence, combining proximity and affinity proteomics, we demonstrate that previously unknown and canonical interactors of CAV complexes can be detected with high specificity, providing a comprehensive strategy for systematic functional analysis. Furthermore, we show that McT1, an abundant cardiac lactate/proton co-transporter, requires CAV3 for stable functional surface expression in human cardiomyocytes. In contrast, CAV3 knockout disrupted McT1-dependent substrate-transport, increased INa,L leading to EAD instability in human cardiomyocytes and proarrhythmogenic reentry in iPSC-CM tissue modeling. These observations highlight the potential of in situ protein labeling and cross-linking approaches to identify new interaction partners and structural interfaces for CAV1 or CAV3 multimers, which are prerequisites to generate assembly models of functional protein complexes in the physiological context of excitable cells. Hence, characterization of novel interactors of CAV1 and CAV3 complexes is central to understand their isoform-specific functions in cardiac cell biology and to develop therapeutic rationales that exploit conserved protein/protein interfaces to augment McT1 function, or to inhibit INa,L causative EAD instability in heart disease.
action potential duration
ascorbate peroxidase engineered X2 variant from soybean
angiotensin II receptor
blue native polyacrylamide gel electrophoresis
data-independent acquisition mass spectrometry
early after depolarization
Eps15 homology domain 2
late sodium current
induced pluripotent stem cell cardiomyocyte
Long-QT syndrome type 9
monocarboxylate transporter type 1
voltage-dependent Na+ channel type 1.5
neonatal rat ventricular myocyte
stable isotope labeling by amino acids in cell culture
nonreceptor protein tyrosine kinase
stimulated emission depletion
We are grateful for excellent technical assistance by Birgit Schumann, Brigitte Korff, Laura Cyganek, Lisa Neuenroth, Thierry Wasselin, Nadine Gotzmann, Martina Grohe, Yvonne Hintz, Lisa Krebs and Yvonne Wedekind; to Steven O. Marx for kindly providing FLAG-NaV1.5 expressing transgenic mice; to Carolin Wichmann for high-pressure freezing and sample preparation for electron tomography; and to Martin Schorb from the Electron Microscopy core facility at the EMBL Heidelberg.
Sources of Funding
This project was funded by Deutsche Forschungsgemeinschaft (DFG) collaborative research units SFB1002 to S.E. Lehnart (projects A09 and S02), to P. Rehling (project A06), to N. Voigt (project A13), to G. Hasenfuss (project D01), to B. Wollnik (D02), to L. Cyganek (project S01); and through SFB1190 to S.E. Lehnart (project P03), and to H. Urlaub (project Z02); and was supported under Germany’s Excellence Strategy (EXC2067/1-390729940). N. Voigt was funded by Deutsche Forschungsgemeinschaft (VO15568/3 and IRTG1816 RP12.3) and the Else-Kröner-Fresenius Foundation (EKFS 2016_A20). S. Brandenburg received financial support through the clinician scientist program Translational Medicine of the University Medical Center Göttingen. E.A. Rog-Zielinska was funded by an Emmy Noether Fellowship by Deutsche Forschungsgemeinschaft (No. 396913060).
Expanded Materials and Methods
Online Figures I–XII
Online Movies I and II
Data Set Excel Tables I–IV
Online Tables I–XIII
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Novelty and Significance
What Is Known?
CAV3 (caveolin3) gene variants can cause a spectrum of striated muscle diseases including cardiac arrhythmias and a cardiomyopathy classified as long-QT syndrome type 9.
Previously, Western blotting showed that CAV1 (caveolin1) is expressed in adult left-ventricular mouse cardiomyocytes.
To date, differential isoform-specific CAV functions through heterologous protein interactions remain unexplored in muscle cells.
What New Information Does This Article Contribute?
Quantitative mass spectrometry profiling revealed that CAV1 is highly abundant and thus ubiquitously expressed, whereas the muscle-specific CAV3 isoform is far less abundant in ventricular mouse cardiomyocytes.
The main cardiac and metabolically relevant transmembrane lactate/proton shuttle, the monocarboxylate transporter McT1, was identified as a novel isoform-specific CAV3 interactor.
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/Cas9 nuclease)-targeted CAV3 knockout in human induced pluripotent stem cell-derived cardiomyocytes disrupts functional McT1 (monocarboxylate transporter type 1) surface expression in the sarcolemmal membrane. CAV3 knockout increased late transmembrane Na+ currents 2-fold leading to arrhythmogenic early afterdepolarizations and reentry arrhythmias in a corresponding multicellular mathematical tissue model.
The findings demonstrate that CAV3 loss-of-function causes multifactorial defects in cardiomyocytes including action potential instability and disrupted lactate/proton co-transport, which is important for cardiac stress adaptation. Identification of isoform-specific heterologous CAV1 and CAV3 protein interactions in cardiomyocytes provides a conceptual framework, which facilitates the systematic exploration of previously unknown interactomic functions throughout cardiac, skeletal, and smooth muscle cell types. Rare pathogenic CAV1 and CAV3 gene variants, as well as CAV1 single nucleotide polymorphisms associated with common arrhythmogenic cardiac phenotypes previously, require future study in the context of cardiac and skeletal muscle dysfunction in patients.