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A Randomized, Placebo-Controlled, Double-Blind Trial of the Effect of Combined Therapy With Deferoxamine and Deferiprone on Myocardial Iron in Thalassemia Major Using Cardiovascular Magnetic Resonance

Originally publishedhttps://doi.org/10.1161/CIRCULATIONAHA.106.648790Circulation. 2007;115:1876–1884

Abstract

Background— Cardiac complications secondary to iron overload are the leading cause of death in β-thalassemia major. Approximately two thirds of patients maintained on the parenteral iron chelator deferoxamine have myocardial iron loading. The oral iron chelator deferiprone has been demonstrated to remove myocardial iron, and it has been proposed that in combination with deferoxamine it may have additional effect.

Methods and Results— Myocardial iron loading was assessed with the use of myocardial T2* cardiovascular magnetic resonance in 167 patients with thalassemia major receiving standard maintenance chelation monotherapy with subcutaneous deferoxamine. Of these patients, 65 with mild to moderate myocardial iron loading (T2* 8 to 20 ms) entered the trial with continuation of subcutaneous deferoxamine and were randomized to receive additional oral placebo (deferoxamine group) or oral deferiprone 75 mg/kg per day (combined group). The primary end point was the change in myocardial T2* over 12 months. Secondary end points of endothelial function (flow-mediated dilatation of the brachial artery) and cardiac function were also measured with cardiovascular magnetic resonance. There were significant improvements in the combined treatment group compared with the deferoxamine group in myocardial T2* (ratio of change in geometric means 1.50 versus 1.24; P=0.02), absolute left ventricular ejection fraction (2.6% versus 0.6%; P=0.05), and absolute endothelial function (8.8% versus 3.3%; P=0.02). There was also a significantly greater improvement in serum ferritin in the combined group (−976 versus −233 μg/L; P<0.001).

Conclusions— In comparison to the standard chelation monotherapy of deferoxamine, combination treatment with additional deferiprone reduced myocardial iron and improved the ejection fraction and endothelial function in thalassemia major patients with mild to moderate cardiac iron loading.

Beta-thalassemia major (TM) affects ≈60 000 births per year worldwide. A mutation in the β-globin gene causes defective erythropoiesis, which in its homozygous form results in a severe anemia, rendering the individual dependent on lifelong blood transfusions. The subsequent development of iron overload can result in cardiomyopathy, and heart failure remains the leading cause of death in TM.1,2 Although treatment with the parenteral iron chelator deferoxamine has improved morbidity and mortality, long-term survival remains poor, with data from the United Kingdom showing 50% of patients dead by the age of 35 years.3

Clinical Perspective p 1884

A number of factors contribute to the high mortality of TM. First, poor compliance with deferoxamine is frequent and has been demonstrated to reduce survival.3,4 Second, despite treatment with deferoxamine, myocardial iron loading is common.5,6 Third, the identification of preclinical cardiomyopathy has proved problematic.7 Left ventricular (LV) dysfunction and heart failure occur late in the disease process, can be resistant to treatment, and carry a poor prognosis.7,8 Improved detection of preclinical cardiomyopathy and the use of better-tolerated chelators as a total or partial replacement for deferoxamine could pave the way for improvement in cardiovascular mortality.

Cardiovascular magnetic resonance (CMR) permits the quantification of myocardial iron through the measurement of T2*, which is highly sensitive to tissue iron concentration and has been validated in the United Kingdom9 and independently in the United States.10 The high reproducibility of T2* measurement makes the technique ideal for longitudinal follow-up studies to determine optimal treatments for reducing myocardial iron.11,12 CMR is established as the gold standard for quantifying LV function and can also assess the effects of iron loading on endothelial function as measured from brachial arterial reactivity,13 which may contribute to cardiovascular pathology in TM.14 Therefore, CMR is the technique of choice for studies of the effects of iron chelators on the cardiovascular system in TM.

Deferiprone is an orally administered iron chelator that has been approved for use as second-line chelation therapy in Europe since 1999. Initial concerns regarding an increased risk of hepatic fibrosis15 have proved unfounded,16 and there are now considerable clinical trial data to suggest that deferiprone is superior in removing myocardial iron compared with deferoxamine5,12; this position has been strengthened with data showing improved outcomes in patients on deferiprone.17,18 Conversely, deferiprone may be less effective in removing hepatic iron than deferoxamine.19 Because of the relative merits of the 2 drugs, the issue of combined therapy has been proposed,20 but few randomized data are available for the cardiovascular system for this maneuver. We therefore initiated a randomized, placebo-controlled, double-blind trial to test the hypothesis that the combined therapy of deferoxamine and deferiprone would result in greater reduction in cardiac iron loading than deferoxamine alone, using myocardial T2* as the primary end point.

Methods

Overall Study Performance

A London-based mobile 1.5-T CMR scanner (Sonata, Siemens, Erlangen, Germany) with specialized cardiovascular capability was transported 3 times to Cagliari, Italy, for this research. Patient recruitment was from 12 thalassemia centers on the island of Sardinia, with patients undergoing local hematology management but traveling to Cagliari for regular visits and CMR scans. After baseline CMR scans, randomization to treatment groups was performed. The study protocol was approved by ethics committees in London and Cagliari. Patient information and consent forms were in Italian, and all patients gave written informed consent.

Study Protocol

The inclusion criteria for patient entry into the trial were as follows: diagnosis of TM currently maintained on subcutaneous deferoxamine monotherapy; age >18 years; maintenance of pretransfusion hemoglobin >9 g/dL; myocardial T2* between 8 and 20 ms (mild to moderate myocardial siderosis); and confirmation of effective contraception throughout the trial. Exclusion criteria were as follows: patients who have previously received deferiprone for a total of >6 months over the last 5 years; patients with previous reaction to deferiprone; neutropenia (absolute neutrophil count <1.5×109/L) at screening; thrombocytopenia (<50×109/L) at screening; liver enzymes >3 times upper limit of normal; and implant incompatible with MR (such as pacemaker), claustrophobia, or other condition making CMR impossible or inadvisable.

The baseline screening was performed by CMR in 167 eligible patients with TM (75 men, 92 women; age range, 18 to 42 years; mean age, 30±5.3 years). Patients without cardiac iron loading (myocardial T2* >20 ms; n=59)9 were excluded. Subjects with severe cardiac siderosis (T2* <8 ms; n=22) were also excluded on the basis that it was not ethical to offer such patients placebo, and they were offered best medical therapy based on current practice according to the decision of the caring hematologist. Patients with mild to moderate cardiac iron loading who satisfied the trial entry criteria (myocardial T2* 8 to 20 ms; n=86) were invited for further detailed assessment by MR. Of these, 74 patients agreed to return for the full MR protocol, and 65 were subsequently randomized to receive either deferoxamine plus deferiprone (combined group; n=32) or deferoxamine plus placebo (deferoxamine group; n=33). The 9 patients who were not randomized consisted of 2 who were unsuitable (1 adverse response to glyceryl trinitrate with frequent ventricular ectopy, 1 atrial fibrillation) and 7 who expressed the concern that although they were willing to participate, they would prefer to defer to participation of others. The overall patient numbers throughout the trial are shown in Figure 1.

Figure 1. Screening, randomization, completion, and withdrawal patient numbers.

The primary end point of the trial was defined as the change between treatment groups from baseline to 12 months in myocardial T2*. The secondary end points were the change from baseline to 12 months in liver T2*; serum ferritin; LV volumes and function; brachial artery reactivity (endothelium dependent and independent); B-type natriuretic peptide (BNP) (Biosite Diagnostics Inc, San Diego, Calif) as a marker of heart failure; compliance with chelation treatments; and adverse events.

Iron Chelation Management and Randomization

Normal clinical management of deferoxamine therapy was performed by the hematologist in accordance with local standard clinical practice and guidelines.21 Current standard treatment for iron loading is subcutaneous deferoxamine (40 to 50 mg/kg body wt) for as many nights per week as the patient will tolerate (ideally a minimum of 5). In addition to the standard deferoxamine treatment, patients were randomized to deferiprone at a dose of 75 mg/kg body wt or placebo with identical appearance. Neither the patients nor the hematologists were aware of the treatment allocation (double-blind).

Cardiovascular Magnetic Resonance

CMR was performed at baseline and after 6 and 12 months of treatment. Myocardial T2* was assessed with the use of a single breath-hold multiecho technique that can be completed in 5 minutes, as previously described.22 In brief, a single 10-mm-thick short-axis midventricular slice of the LV was acquired at 8 echo times (2.6 to 16.7 ms, with 2.02-ms increments) with standard shimming in a single breath-hold. For analysis, a full-thickness region of interest was chosen in the LV septum. The signal intensity of this region was measured for each image with the use of in-house designed software (CMRtools, Cardiovascular Imaging Solutions, London, UK). Ventricular volumes were determined with the use of steady state free precession cines, with contiguous short-axis slices from base to apex, as previously described.23 Ventricular volumes were also analyzed with the use of CMRtools. For the assessment of endothelial function, flow-mediated dilatation (FMD) of the brachial artery was examined by a validated CMR technique.13 This was performed at baseline and 12 months only for logistical reasons. A 2-cm surface coil was placed over the brachial artery. The brachial artery was occluded for 5 minutes by inflating a blood pressure cuff on the forearm to 20 mm Hg above systolic pressure. On release, the artery was scanned immediately and at 1 to 5 minutes after cuff deflation. The cross-sectional area of the artery was calculated at baseline and at maximal dilatation, and the resulting FMD was expressed as percent change in area. For assessment of endothelium-independent dilatation, the percent change in brachial artery area was measured before and 3 minutes after sublingual administration of glyceryl trinitrate (400 μg).

Serum Ferritin

Serum ferritin was measured at baseline and every 2 months. Serum was separated, labeled, and stored frozen at −20°C and was measured by microparticle enzyme immunoassay (Abbott AXSYM System).

Safety Assessments

Patients were monitored weekly for absolute neutrophil count and any adverse events. Serum alanine aminotransferase levels were measured every 2 months, serum zinc levels were measured at baseline and every 6 months, and serum creatinine levels were measured at baseline, 6 months, and 12 months.

Statistical Analysis

Power calculations showed that the study would have an 80% power at a significance level of 0.05 to detect a change in the primary end point of myocardial T2* of 0.75 SD with a sample size of 55 patients, which, allowing for a 15% patient dropout, indicated that we should randomize 65 patients. Differences between groups for all continuous parameters at baseline were analyzed by the unpaired t test. Because tissue iron is related linearly to the inverse of T2*, this measure was log-transformed before analysis for normalization. It was also necessary to log-transform serum ferritin levels to achieve normality. Data are presented as mean±SD, except for the T2* and ferritin data, which use the geometric mean (anti-log of the mean of the log data) plus or minus the coefficient of variation (CV), equivalent to the variance of the mean in log scale. Between-group analysis from baseline to 12 months was performed with the use of a mixed-effects model, with baseline readings and treatment group entered as fixed effects and patients considered a random effect. Trend analysis over time within groups for continuous parameters was performed with a mixed model with time as a fixed effect. In the case of FMD, in which measurement at only 2 time points was available, ANCOVA was used with treatment, and baseline measures were entered as covariates. Proportions of patients between the treatment groups were compared by Fisher exact test. Deferoxamine compliance was calculated as the percentage of completed infusions, as determined by the Crono pumps, divided by the number of infusions prescribed. Deferiprone/placebo compliance was measured through pill counting at the bimonthly visits. Statistical analysis was performed with the use of SPSS version 10.0 (SPSS, Inc, Chicago, Ill) and STATA 9.2 (StataCorp, College Station, Tex). A probability value of 0.05 was the threshold used for statistical significance.

The authors had full access to and take full responsibility for the integrity of the data. All authors have read and agree to the manuscript as written.

Results

Patient Characterization

The baseline characteristics are shown in the Table. The groups were well matched for all measures at baseline, including the primary end point of myocardial siderosis.

Descriptive Statistics of the Treatment Groups at Baseline

CombinedDeferoxamineP
Data are shown as n (%) or mean±SD, except for myocardial and hepatic T2* and serum ferritin, which are shown as geometric mean and CV. Normal values are shown in square brackets where appropriate. NA indicates not applicable.
No. randomized32 (49)33 (51)NA
Age, y28.8±4.228.7±5.30.9
Gender
    Male14 (44)13 (39)0.5
    Female18 (56)20 (61)
Italian ethnicity32 (100)33 (100)NA
Body surface area, m21.53±0.151.56±0.160.5
Deferoxamine dose, mg/kg per day40.6±13.2 (5 d/wk)40.5±14.0 (5 d/wk)1.0
CMR measures
    Myocardial T2*, ms [>20]11.7 (0.08)12.4 (0.11)0.3
    Liver T2*, ms [>19]4.9 (0.52)4.2 (0.62)0.5
    LV end-diastolic volume, mL [M: 77–195; F: 52–141]125±30.5128±37.40.3
    LV end-systolic volume, mL [M: 19–72; F: 13–51]43.3±16.145.9±18.70.4
    LV ejection fraction, % [>56%]65.8±6.264.7±6.50.5
Brachial artery reactivity, % area change
    FMD [>11.5%]10.0±7.710.0±9.51.0
    Glyceryl trinitrate–induced dilatation [>44%]47.6±18.250.9±18.90.5
Blood measures
    Transfusional red blood cell input, mL/kg per year133.4±34.9130.2±38.60.7
    Hemoglobin, g/L106±9.6102±9.50.1
    Total white blood cell count, ×109/L7.7±3.268.0±3.670.8
    Absolute neutrophil count, ×109/L4.1±1.84.4±1.50.5
    Platelets, ×109/L277±124296±1480.7
Hepatitis C positive
    Yes23 (72)26 (79)0.4
    No9 (28)7 (21)
Biochemistry
    Serum ferritin, μg/L [M: 5–104; F: 4–254]1574 (11)1379 (10)0.5
    Alanine aminotransferase, IU/L [5–35]54±4866±430.3
    BNP, pmol/L [<100]23.4±25.324.17±26.30.9
    Creatinine, mg/dL [0.6–1.2]0.77±0.210.74±0.230.6
    Zinc, μg/dL [70–130]94.5±16.698.5±17.20.4

Deferoxamine Dosing

At baseline, both groups were well matched for the prescribed dose of deferoxamine (Table). The mean dose of deferoxamine in all randomized patients (deferoxamine and combined groups) before trial entry was 36.4±11.1 mg/kg per day for 5.5 d/wk (equivalent to 40.5 mg/kg for 5 d/wk). This is in accord with the 40 to 50 mg/kg per day for 5 d/wk from clinical recommendations.21 During the trial, the dose prescribed for deferoxamine in the deferoxamine group was equivalent to 43.4 mg/kg per day for 5 d/wk (2.5±9.2 mg/kg per day; P=0.1 versus pretrial maintenance dose for the deferoxamine group only). In comparison, the dose prescribed in the combined group during the trial was significantly lower at 34.9 mg/kg per day for 5 days (P=0.02 versus deferoxamine group).

Deferiprone Dosing

At baseline, no patient was taking deferiprone. During the trial, the dose prescribed for deferiprone was 75 mg/kg per day for all patients.

Compliance

Compliance with deferoxamine was similar in both groups (combined 91.4±12.7% versus deferoxamine 92.6±12.7%; P=0.7). Compliance with deferiprone was less than compliance with placebo (82.4±18.1% versus 89.8±7.2%; P=0.04).

Myocardial Iron

In the combined treatment group, myocardial T2* improved significantly (indicating reduced myocardial iron) from a baseline of 11.7 ms to 14.7 ms at 6 months and 17.7 ms at 12 months (ratio of geometric means 1.50; CV 13%; P<0.001; Figure 2). In the deferoxamine group, myocardial T2* changed from a baseline of 12.4 ms to 14.5 ms at 6 months and 15.7 ms at 12 months (ratio 1.24; CV 16%; P=0.001). The between-group difference was significantly in favor of the combined treatment group, with an estimate of a 10% increase in the combined group compared with the deferoxamine group (95% CI, 2% to 19%; P=0.02).

Figure 2. Myocardial T2* showed a significantly larger improvement from baseline in the combined group than in the deferoxamine group. The vertical axis shows the geometric mean of T2*. Standard error bars are shown.

Liver Iron

In the combined group, liver T2* improved significantly from a baseline of 4.9 ms to 9.5 ms at 6 months and 10.7 ms at 12 months (ratio of geometric means 2.07; CV 37%; P<0.001; Figure 3). In the deferoxamine group, there was a significant change in liver T2* from a baseline of 4.2 ms to 4.9 ms at 6 months and 5.0 ms at 12 months (ratio 1.20; CV 50%; P=0.01). The between-group difference (39%) was significantly in favor of the combined treatment group (95% CI, 20% to 61%; P<0.001).

Figure 3. Liver T2* showed a significantly larger improvement from baseline in the combined group compared with the deferoxamine group. The vertical axis shows the geometric mean of T2*. Standard error bars are shown.

Cardiac Function and Volumes

There was a significant difference between treatment groups with respect to changes in LV ejection fraction over 12 months. In the combined group, LV ejection fraction increased from 65.8±6.2% at baseline to 68.4±4.7% at 12 months, and in the deferoxamine group, LVEF increased from 64.7±6.5% to 65.3±6.0% (absolute percent difference between groups, 1.17%; 95% CI, 0.0% to 2.35%; P=0.05; Figure 4). There was no significant difference between treatment groups in terms of end-diastolic volume and end-systolic volume. End-diastolic volume decreased from 124.9±30.5 to 119.1±31.8 mL over 12 months in the combined group and changed from 127.5±37.4 mL at baseline to 126.4±35.5 mL in the deferoxamine group (difference between groups, −3.83 mL; 95% CI, −20.23 to 12.55; P=0.7). End-systolic volume decreased from 43.3±16.1 to 38.3±13.3 mL in the combined group, and end-systolic volume changed from 46±18.9 to 44.7±17.2 mL over 12 months in the deferoxamine group (P=0.3). There was a significant correlation between improved ejection fraction and baseline myocardial T2* in all study patients with baseline T2* <10 ms (r=0.67, P<0.01) but no significant correlation in the entire study group. For the 8 patients treated with deferiprone with baseline myocardial T2* <10 ms, the ejection fraction rose by 4.9% over 12 months. Seven patients were treated with placebo with baseline myocardial T2* <10 ms, and in this group the ejection fraction fell by 0.1% over 12 months (P=0.05 for the difference between groups).

Figure 4. There was a greater improvement from baseline in LV ejection fraction in the combined group compared with the deferoxamine group. Standard error bars are shown.

Brachial Artery Reactivity

In the combined treatment group, endothelial-dependent FMD improved significantly (baseline 10.0±7.7% versus 18.8±9.3% at 12 months; P<0.001; Figure 5). There was no significant change in FMD in the deferoxamine group (baseline 10.0±9.5% versus 13.3±9.8% at 12 months; P=0.1). The between-group difference was significantly in favor of the combined treatment group, with a treatment effect of 5.9% (95% CI, 0.99% to 10.8%; P=0.02). There was no significant change in endothelium-independent glyceryl trinitrate–induced dilatation in either the combined group (47.6±18.2% baseline versus 50.9±17.7% at 12 months; P=0.3) or deferoxamine group (50.9±18.9% baseline versus 49.6±24.6% at 12 months; P=0.7).

Figure 5. There was significant improvement in endothelial function in the combined group but not in the deferoxamine group. Standard error bars are shown. Lower limit of normal FMD (11.5%) is illustrated by dashed line.

Serum Ferritin

In the combined treatment group, there was a significant reduction in ferritin, from 1574 μg/L at baseline to 598 μg/L at 12 months (CV 14%; P<0.001; Figure 6). There was also significant change in the ferritin of the deferoxamine group over 12 months, from 1379 μg/L at baseline to 1146 μg/L at 12 months (CV 11%; P=0.01). The between-group difference was significantly in favor of the combined treatment group (−40%; 95% CI, −48% to −28%; P<0.001).

Figure 6. There was a significant reduction in serum ferritin in the combined group but not in the deferoxamine group. The vertical axis shows the geometric mean of ferritin.

B-Type Natriuretic Peptide

There was no significant change in BNP in either the combined group (23.4±25.3 pmol/L at baseline versus 26.2±32.7 pmol/L at 12 months; P=0.5) or the deferoxamine group (24.7±26.6 pmol/L at baseline versus 29.6±23.2 pmol/L at 12 months; P=0.4).

Other Laboratory Measures

There was no significant change in alanine aminotransferase over the trial period in either the combined group (54±48 U/L at baseline versus 55±36U/L at 12 months; P=0.7) or the deferoxamine group (66±43 U/L at baseline versus 60±34 U/L at 12 months; P=0.8). There was no significant difference between combined and deferoxamine groups in the change of zinc level at 12 months (−12.8±14.5 versus −7.6±15.7 μg/dL; P=0.2). There was no significant difference between combined and deferoxamine groups in the change in creatinine at 12 months (0.04±0.17 versus 0.05±0.17 mg/dL; P=1.0).

Adverse Events and Subject Withdrawals

Gastrointestinal symptoms (nausea, vomiting, or abdominal pain) were the most common adverse event, occurring in 38% of patients in the combined group and 24% of patients in the deferoxamine group (P=0.2). These were generally mild in severity, although they were more likely to be recurrent (>2 episodes) in the combined group compared with the deferoxamine group (19% versus 3%; P=0.05). Other adverse events included reactions at the deferoxamine infusion site, occurring in 3% of the combined group and 6% of the deferoxamine group. Joint problems, including pain and/or swelling, occurred in 9% of the combined patients and in 18% of the deferoxamine group (P=0.3). In the combined group, there was 1 episode of agranulocytosis, which recovered without complication with the use of granulocyte colony-stimulating factor. There were 2 episodes of neutropenia (1.0 to 1.5×109/L) in the combined group. Both episodes resolved after discontinuation of the drug, and neither of these subjects was withdrawn from the study. In total, there were 7 patient withdrawals: 6 in the first month of treatment and 1 after 6 months of treatment; there were 4 in the combined group (agranulocytosis [n=1], reduction of myocardial T2* to <8 ms [n=1], gastrointestinal symptoms [n=1], and personal reasons [n=1]) and 3 in the deferoxamine group (atrial fibrillation [n=1] and personal reasons [n=2]).

Discussion

The cardiomyopathy resulting from iron toxicity is the single most important cause of death in individuals with TM.1,2,4 Despite treatment with the most widely used iron chelator deferoxamine, a significant proportion of patients have myocardial iron loading.5,6 Therefore, a logical step toward an improvement in cardiac mortality is to optimize the treatment of myocardial siderosis. Although deferoxamine is an important chelator24 with a very high affinity for iron, it is a large and relatively lipophobic molecule that does not readily cross cell membranes. In contrast, deferiprone is small and lipophilic and readily crosses cell membranes, thereby gaining access to intracellular iron.25 These differential properties form the basis of the “shuttle hypothesis,” which proposes that combination therapy has additive or synergistic effects.26 There is evidence for this in vitro,27 and a number of clinical studies have concluded that combined therapy offers additional benefit in terms of iron loading and cardiac function.28,29 Despite these studies, widespread acceptance of the use of combination therapy has not occurred because prospective, randomized, placebo-controlled data have not been available. Our study provides such evidence for the first time. Our study shows that the addition of deferiprone to deferoxamine therapy has favorable effects in the treatment of iron overload in TM and in particular shows that combination therapy results in a significant relative reduction in myocardial siderosis. Although there are currently no published data on the direct prognostic significance of myocardial T2*, there are data demonstrating the adverse effects of a low T2*. Subjects with a very low myocardial T2* are at risk of symptomatic heart failure and ventricular arrhythmias.6,10 Myocardial T2* improves in concert with cardiac function in response to intensification of chelation therapy in severe myocardial iron loading.11 It is therefore highly probable that an increase in T2* is associated with an improvement in cardiovascular outcome.

A number of changes in secondary outcome measures were also more favorable in the combined group. The relative improvement in liver iron and serum ferritin was significantly greater in the combined group. The superior effects on LV ejection fraction are in agreement with prior combination trial data28,29 and are most obvious in patients with the highest myocardial iron burden (myocardial T2* <10 ms). These patients have LV ejection fractions that are subnormal relative to thalassemia patients with no myocardial iron loading,30 and therefore their rise in ejection fraction represents relief of subclinical LV dysfunction.12 Of additional considerable interest was the significant relative improvement in endothelial function in the combined treatment group. Endothelial dysfunction has previously been described in patients with TM.14 Our results support this finding with a mean FMD of the baseline cohort of 10.0±8.6% (normal mean value as determined by CMR, 15.5±2.0%).31 Iron-mediated endothelial dysfunction may be mediated either directly through the inactivation of endothelium-derived nitric oxide or indirectly through the promotion of reactive oxygen species formation.32 This study demonstrates for the first time that intensification of chelation in patients with TM results in large improvements in endothelial function. In TM, endothelial dysfunction has been linked to increased arterial stiffness.14 This is highly relevant to the management of those patients in whom the interactions between vasculature and ventricle are likely to play a significant role in the development and progression of heart failure. Relief of endothelial dysfunction in patients with heart failure might therefore be beneficial. Although optimization of chelation therapy improves endothelial function, there are other therapeutic options, such as isosorbide dinitrate, that stimulate nitric oxide signaling,33 which might provide additional protection from heart failure and cardiovascular complications.

The baseline cross-sectional findings of the cohort screened for this study have been reported previously.6 These findings showed that BNP was inadequate as a predictor of myocardial iron loading. The present study now demonstrates in addition that serial measurements of BNP show no significant change in either of the treatment groups, despite large improvements in myocardial siderosis in the combined group. These results suggest a very limited role for BNP as a convenient surrogate marker for myocardial iron.

Although compliance to deferoxamine was very similar in both groups, the actual prescribed dose was significantly lower in the combined group. Thus, despite lower deferoxamine dosing in the combined group, these subjects still demonstrated improved iron clearance. The likely explanation for the disparity in dosing between groups is that hematologists would be expected to lower the dose of deferoxamine in response to falling serum ferritin, which occurred more frequently in the combined group (ferritin <500 μg/L was seen in 1 patient in each group at baseline but at 6 months occurred in 11 of 32 [34%] in the combined group but in only 1 of 33 [3%] in the deferoxamine group; P=0.001).

Compliance with deferiprone was lower than compliance with deferoxamine (82.4% versus 89.8%; P=0.04). On further analysis of individual cases, it was clear that the majority of cases of poor chelation (compliance <75%; n=8) were associated with adverse events. There were 2 cases of transient neutropenia, 3 cases of elevated alanine aminotransferase, and 2 cases of gastrointestinal intolerance, and 1 subject requested a drug “vacation.” In all cases, adverse effects in response to deferiprone were transient and responded to either dose reduction or temporary drug cessation. These adverse events have been documented in previous studies of deferiprone monotherapy and combined therapy.12,20,28,29 Overall, in the entire combination treatment group, there were 2 cases of neutropenia and 1 case of agranulocytosis. It is not clear whether this represents a higher than expected level of toxicity because of the small number involved. Therefore, although the superior efficacy of combined therapy has been clearly demonstrated, the incidence of adverse events requires further scrutiny.

Limitations

The results of the present study apply to adults with mild to moderate myocardial iron loading and may not necessarily be applicable to patients with severe cardiac siderosis or symptomatic heart failure. Combined therapy was better than monotherapy as currently practiced (40 mg/kg per day for 5 d/wk) and was a safe, consistent, and well-tolerated mechanism to ensure negative iron balance in the liver, heart, and endothelium; similar data do not exist for higher-dose deferoxamine therapy, which is an alternative strategy. However, a further advantage of combined treatment is that the deferoxamine treatment can be administered less often and for a shorter duration, which helps patient compliance.

Conclusions

In comparison to the standard chelation therapy of maintenance subcutaneous deferoxamine, combined therapy including deferiprone demonstrates superior reduction in myocardial iron and improved ventricular and endothelial function. This therapy is likely to translate into an improvement in cardiovascular mortality and should be considered in patients whose deferoxamine monotherapy fails to achieve adequate control of myocardial iron.

We would like to thank the following colleagues from the Sardinian thalassemia centers involved in the trial: Simona Piras, Martina Pibiri, S. Mulas (Ospedale Civile, Alghero); G. Tocco (Ospedale Sirai, Carbonia); N. Landis (Ospedale F. lli Crobu, Iglesias); I. Contu, P. Cannas (Ospedale Civile, Lanusei); G. Puggioni (Ospedale San Francesco, Nuoro); A. Zuccarelli (Ospedale Civile, Olbia); A. Carta (Ospedale Civile San Martino, Oristano); G. Bertrand (Ospedale A. Segni, Ozieri); M.G. Batzella (Ospedale Civile, San Gavino); G. Sechi (Centro Trasfusionale, Sassari); D. Gallisai (Clinica Pediatrica, Sassari, and “Fondazione L. Giambrone” Sassari). We also thank Mohammed Khan for statistical advice and analysis and the following colleagues for their important contributions: David Firmin, Taigang He, Steve Collins, Tim Cannell, Ray Hughes, Niall Keenan, and Craig Broberg.

Sources of Funding

Funding was received from CORDA, Royal Brompton, and Harefield Hospital Charitable Funds, the Cooley’s Anemia Foundation, Apotex, the UK Thalassemia Society, and the University College London Special Trustees Charity. The funding sources played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Disclosures

Dr Pennell had full access to all the data in the study and had final responsibility for the decision to submit for publication. The authors are solely responsible for the conduct, data storage, data analysis, and reporting of this trial. Dr Pennell is a consultant to, has received speaker’s honoraria and research support from, and has participated in chelation drug research with Apotex. He is also a consultant to and is participating in chelation drug research with Novartis. He is a consultant to Siemens Medical Solutions and a director of Cardiovascular Imaging Solutions. Dr Galanello has received speaker’s honoraria and research support from Apotex and Novartis. Ms Smith is a consultant to and is participating in chelation drug research with Novartis. Dr Walker has received research support from Novartis and speaker’s honoraria from Apotex. Dr Westwood has received speaker’s honoraria from Apotex. Drs Assomull and Nair are supported by the British Heart Foundation. The remaining authors report no conflicts.

Footnotes

Correspondence to Dr Dudley Pennell, Professor of Cardiology, Royal Brompton Hospital, Sydney St, London SW3 6NP, UK. E-mail

References

  • 1 Olivieri NF, Nathan DG, MacMillan JH, Wayne AS, Liu PP, McGee A, Martin M, Koren G, Cohen AR. Survival in medically treated patients with homozygous beta-thalassemia. N Engl J Med. 1994; 331: 574–578.CrossrefMedlineGoogle Scholar
  • 2 Borgna-Pignatti C, Rugolotto S, De Stefano P, Zhao H, Cappellini MD, Del Vecchio GC, Romeo MA, Forni GL, Gamberini MR, Ghilardi R, Piga A, Cnaan A. Survival and complications in patients with thalassemia major treated with transfusion and deferoxamine. Haematologica. 2004; 89: 1187–1193.MedlineGoogle Scholar
  • 3 Modell B, Khan M, Darlison M. Survival in beta thalassaemia major in the UK: data from the UK Thalassaemia Register. Lancet. 2000; 355: 2051–2052.CrossrefMedlineGoogle Scholar
  • 4 Piga A, Longo F, Consolati A, De Leo A, Carmellino L. Mortality and morbidity in thalassaemia with conventional treatment. In: Proceedings of the Third International Conference on Bone Marrow Transplantation in Thalassaemia. Bone Marrow Transplant. 1997; 19 (suppl 2): 11–13.Google Scholar
  • 5 Anderson LJ, Wonke B, Prescott E, Holden S, Walker JM, Pennell DJ. Comparison of effects of oral deferiprone and subcutaneous desferrioxamine on myocardial iron levels and ventricular function in beta thalassemia. Lancet. 2002; 360: 516–520.CrossrefMedlineGoogle Scholar
  • 6 Tanner MA, Galanello R, Dessi C, Westwood MA, Smith GC, Nair SV, Anderson LJ, Walker JM, Pennell DJ. Myocardial iron loading in patients with thalassemia major on deferoxamine chelation. J Cardiovasc Magn Reson. 2006; 8: 543–547.CrossrefMedlineGoogle Scholar
  • 7 Jessup M, Manno CS. Diagnosis and management of iron-induced heart disease in Cooley’s anemia. Ann NY Acad Sci. 1998; 850: 242–250.CrossrefMedlineGoogle Scholar
  • 8 Kremastinos DT, Tsetsos GA, Tsiapras DP, Karavolias GK, Ladis VA, Kattamis CA. Heart failure in beta thalassemia: a 5-year follow-up study. Am J Med. 2001; 111: 349–354.CrossrefMedlineGoogle Scholar
  • 9 Anderson LJ, Holden S, Davies B, Prescott E, Charrier C, Bunce NH, Firmin DN, Porter JB, Wonke B, Walker JM, Pennell DJ. Cardiovascular T2* (T2 star) magnetic resonance for the early diagnosis of myocardial iron overload. Eur Heart J. 2001; 22: 2171–2179.CrossrefMedlineGoogle Scholar
  • 10 Wood JC, Tyszka JM, Carson S, Nelson MD, Coates TD. Myocardial iron loading in transfusion-dependent thalassemia and sickle cell disease. Blood. 2004; 103: 1934–1936.CrossrefMedlineGoogle Scholar
  • 11 Anderson LJ, Westwood MA, Holden S, Davis B, Prescott E, Wonke B, Porter JB, Walker JM, Pennell DJ. Myocardial iron clearance during reversal of siderotic cardiomyopathy with intravenous desferrioxamine: a prospective study using T2* cardiovascular magnetic resonance. Br J Haematol. 2004; 127: 348–355.CrossrefMedlineGoogle Scholar
  • 12 Pennell DJ, Berdoukas V, Karagiorga M, Ladis V, Piga A, Aessopos A, Gotsis ED, Tanner MA, Smith GC, Westwood MA, Wonke B, Galanello R. Randomized controlled trial of deferiprone or deferoxamine in beta-thalassemia major patients with asymptomatic myocardial siderosis. Blood. 2006; 107: 3738–3744.CrossrefMedlineGoogle Scholar
  • 13 Sorenson MB, Collins P, Ong PJL, Webb CM, Hayward CS, Asbury EA, Gatehouse PD, Elkington AG, Yang GZ, Kubba A, Pennell DJ. Long term use of contraceptive depot medroxyprogesterone acetate in young women impairs arterial endothelial function assessed by cardiovascular magnetic resonance. Circulation. 2002; 106: 1646–1651.LinkGoogle Scholar
  • 14 Cheung YF, Chan GC, Ha SY. Arterial stiffness and endothelial function in patients with beta-thalassemia major. Circulation. 2002; 106: 2561–2566.LinkGoogle Scholar
  • 15 Olivieri NF, Brittenham GM, McLaren CE, Templeton DM, Cameron RG, McClelland RA, Burt AD, Fleming KA. Long-term safety and effectiveness of iron-chelation therapy with deferiprone for thalassemia major. N Engl J Med. 1998; 339: 417–423.CrossrefMedlineGoogle Scholar
  • 16 Wanless IR, Sweeney G, Dhillon AP, Guido M, Piga A, Galanello R, Gamberini MR, Schwartz E, Cohen AR. Lack of hepatic fibrosis during long-term therapy with deferiprone in subjects with transfusion-dependent beta-thalassemia. Blood. 2002; 100: 1566–1569.CrossrefMedlineGoogle Scholar
  • 17 Piga A, Caglioti C, Fogliacco E, Tricta F. Comparative effects of deferiprone and deferoxamine on survival and cardiac disease in patients with thalassaemia major: a retrospective analysis. Haematologica. 2003; 88: 489–496.MedlineGoogle Scholar
  • 18 Borgna-Pignatti C, Cappellini MD, De Stefano P, Del Vecchio GC, Forni GL, Gamberini MR, Ghilardi R, Piga A, Romeo MA, Zhao H, Cnaan A. Cardiac morbidity and mortality in deferoxamine- or deferiprone-treated patients with thalassemia major. Blood. 2006; 107: 3733–3737.CrossrefMedlineGoogle Scholar
  • 19 Caro J, Huybrechts KF, Green TC. Estimates of the effect on hepatic iron of oral deferiprone compared with subcutaneous desferrioxamine for treatment of iron overload in thalassemia major: a systematic review. BMC Blood Disord. 2002; 2: 4.MedlineGoogle Scholar
  • 20 Wonke B, Wright C, Hoffbrand AV. Combined therapy with deferiprone and desferrioxamine. Br J Haematol. 1998; 103: 361–364.CrossrefMedlineGoogle Scholar
  • 21 Porter JB. Practical management of iron overload. Br J Haematol. 2001; 115: 239–252.CrossrefMedlineGoogle Scholar
  • 22 Westwood M, Anderson LJ, Firmin DN, Gatehouse PD, Charrier CC, Wonke B, Pennell DJ. A single breath-hold multiecho T2* cardiovascular magnetic resonance technique for diagnosis of myocardial iron overload. J Magn Reson Imaging. 2003; 18: 33–39.CrossrefMedlineGoogle Scholar
  • 23 Maceira AM, Prasad SK, Khan M, Pennell DJ. Normalized left ventricular systolic and diastolic function by steady state free precession cardiovascular magnetic resonance. J Cardiovasc Magn Reson. 2006; 8: 417–426.CrossrefMedlineGoogle Scholar
  • 24 Brittenham GM, Griffith PM, Nienhuis AW, McLaren CE, Young NS, Tucker EE, Allen CJ, Farrell DE, Harris JW. Efficacy of deferoxamine in preventing complications of iron overload in patients with thalassemia major. N Engl J Med. 1994; 331: 567–573.CrossrefMedlineGoogle Scholar
  • 25 de Franceschi L, Shalev O, Piga A, Collell M, Olivieri O, Corrocher R, Hebbel RP, Brugnara C. Deferiprone therapy in homozygous human beta-thalassemia removes erythrocyte membrane free iron and reduces KCl cotransport activity. J Lab Clin Med. 1999; 133: 64–69.CrossrefMedlineGoogle Scholar
  • 26 Grady RW, Berdoukas EA, Rachmilewitz, Giardina PJ. Optimizing chelation therapy: combining deferiprone and desferrioxamine. Blood. 1999; 96: 604a.Google Scholar
  • 27 Link G, Konjin AM, Breuer W, Cabantchik ZI, Hershko C. Exploring the “iron shuttle” hypothesis in chelation therapy: effects of combined deferoxamine and deferiprone treatment in hypertransfused rats with labelled iron stores and in iron-loaded rat heart cells in culture. J Lab Clin Med. 2001; 138: 130–138.CrossrefMedlineGoogle Scholar
  • 28 Origa R, Bina P, Agus A, Crobu G, Defraia E, Dessi C, Leoni G, Muroni PP, Galanello R. Combined therapy with deferiprone and desferrioxamine in thalassemia major. Haematologica. 2005; 90: 1309–1314.MedlineGoogle Scholar
  • 29 Daar S, Pathare AV. Combined therapy with desferrioxamine and deferiprone in beta thalassaemia major patients with transfusional iron overload. Ann Hematol. 2006; 85: 315–319.CrossrefMedlineGoogle Scholar
  • 30 Westwood MA, Anderson LJ, Maceira AM, Shah FT, Prescott E, Porter JB, Wonke B, Walker JM, Pennell DJ. Normalised left ventricular volumes and function in thalassaemia major patients with normal myocardial iron. J Magn Reson Imaging. In press.Google Scholar
  • 31 Wiesmann F, Petersen SE, Leeson PM, Francis JM, Robson MD, Wang Q, Choudhury R, Channon KM, Neubauer S. Global impairment of brachial, carotid, and aortic vascular function in young smokers. J Am Coll Cardiol. 2004; 44: 2056–2064.CrossrefMedlineGoogle Scholar
  • 32 Duffy SJ, Biegelsen ES, Holbrook M, Russell JD, Gokce N, Keaney JF, Vita JA. Iron chelation improves endothelial function in patients with coronary artery disease. Circulation. 2001; 103: 2799–2804.CrossrefMedlineGoogle Scholar
  • 33 Sydow K, Daiber A, Oelze M, Chen Z, August M, Wendt M, Ullrich V, Mulsch A, Schulz E, Keaney JF, Stamler JS, Munzel T. Central role of mitochondrial aldehyde dehydrogenase and reactive oxygen species in nitroglycerin tolerance and cross-tolerance. J Clin Invest. 2004; 113: 352–354.CrossrefMedlineGoogle Scholar
circulationahaCirculationCirculationCirculation0009-73221524-4539Lippincott Williams & Wilkins
CLINICAL PERSPECTIVE10042007

Thalassemia is the most common single gene disorder worldwide. Lifelong transfusion dependence causes damaging tissue iron accumulation. Excess iron can be removed with chelation, and deferoxamine has been used since the late 1960s, but this is difficult, typically requiring a subcutaneous injection schedule for 8 hours for 5 nights per week to achieve iron balance. Deferoxamine improves life expectancy, but in recent cohorts half of the patients still die before the age of 35 years, with 70% of patients dying of heart failure. Addressing this premature cardiac mortality was difficult until 2001, when a new cardiac iron measurement technique based on magnetic relaxation of myocardial T2* was reported. Myocardial T2* is normally 40 ms, with a lower limit of normal of 20 ms. Increased iron causes faster relaxation that shortens the T2* value to <20 ms, with values <10 ms indicating severe myocardial siderosis, which is strongly associated with heart failure. Research is now focused on the manner in which to treat patients effectively once myocardial siderosis is identified. Deferiprone is a small neutral molecule that has good access to myocyte iron and is known to be effective in removing cardiac iron as monotherapy. In the present study, deferiprone was added to deferoxamine in a randomized placebo-controlled trial. Myocardial iron improved significantly more with the combination than with deferoxamine alone. Therefore, combination therapy is superior to deferoxamine monotherapy in removing myocardial iron, and it should be considered for patients with myocardial siderosis and may be valuable in more severe myocardial siderosis associated with heart failure.

Clinical trial registration information—URL: http://www.clinicaltrials.gov. Unique identifier: NCT00103753.