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Mineralocorticoid Receptor Activation and Mineralocorticoid Receptor Antagonist Treatment in Cardiac and Renal Diseases

Originally publishedhttps://doi.org/10.1161/HYPERTENSIONAHA.114.04488Hypertension. 2015;65:257–263

Cardiac and renal diseases remain major challenges for healthcare systems in developed countries and commonly coexist, with a disorder in the heart or kidney often leading to secondary dysfunction or injury in the other organ. Indeed, the term cardiorenal syndrome has been introduced to describe the broad spectrum of disease involving both the heart and kidneys.1

Aldosterone is a steroid hormone with mineralocorticoid activity, produced primarily in the glomerular zone of the adrenal cortex.2 Aldosterone fulfills its major physiological function of maintaining sodium and potassium balance and blood pressure control by binding to the mineralocorticoid receptor (MR) in the connecting tubule and cortical collecting duct in the kidneys, thereby increasing sodium reabsorption and potassium secretion. There is a growing body of evidence for a broader role of aldosterone and MR activation in the pathophysiology of cardiovascular and renal disease.3,4

This article focuses on our knowledge and understanding of the direct roles of aldosterone and MR activation in the heart and kidneys, including common pathophysiological mechanisms in both organs and implications for clinical use of MR antagonists (MRAs) in the treatment of cardiac and renal diseases.

MR Signaling in the Kidneys and Heart

MR expression has been demonstrated in vivo in vascular endothelial cells and vascular smooth muscle cells of interlobar arteries in mouse kidneys and ex vivo in cultured podocytes, mesangial cells, and renal fibroblasts.3 The MR is also expressed in multiple cell types in the heart, including cardiomyocytes, coronary endothelial and vascular smooth muscle cells, fibroblasts, and inflammatory cells, such as macrophages.5

Aldosterone and the glucocorticoid cortisol bind to the MR with similar affinities. Plasma concentrations of glucocorticoids are 100- to 1000-fold higher than those of aldosterone. Overstimulation of the MR is prevented by the coexpression of 11β-hydroxysteroid dehydrogenase type 2 (11-BHSD2). This enzyme converts cortisol into cortisone, which has a lower affinity for the MR. 11-BHSD2 activity has been demonstrated in both the kidneys and heart;6,7 however, expression of 11-BHSD2 varies in different cell types, with some cells, such as cardiomyocytes, having very low levels of 11-BHSD2. There is not yet consensus on whether aldosterone or cortisol activates the MR in these cells, but direct effects of aldosterone have still been observed, suggesting mechanisms yet to be determined fully may operate to allow the binding of aldosterone to the MR.8,9

Once aldosterone binds to the MR, the hormone–receptor complex dimerizes, migrates into the nucleus, and binds to a specific DNA sequence, triggering the transcription of target genes. It takes ≈1 hour for the classic genomic action of aldosterone to start to have a biological effect, with complete changes in gene expression not apparent for hours or days.10 A second, more rapid, nongenomic pathway, the effects of which are short-lived, has also been described, although the physiological and clinical relevance of this pathway remains to be established.11,12

The principal functional role of the MR in normal kidneys is to control sodium reabsorption and potassium secretion.3 The function of the MR in the healthy heart is not fully understood, but may include regulation of cardiomyocyte growth and cardiac electrophysiology.6,13

Key evidence for the role of the MR in the pathophysiology of cardiac and renal diseases has come from cell-specific overexpression and deletion studies. In mouse models of chronic pressure overload or myocardial infarction (MI), deletion or inactivation of the MR gene attenuated left ventricular dilatation, cardiac hypertrophy, and development of heart failure (HF), whereas overexpression of the MR in cardiomyocytes induced ventricular remodeling, development of HF, and proarrhythmogenic effects.1417 Expression of both the MR and 11-BHSD2 is upregulated in rats post-MI and in response to a high-salt diet.18,19 In patients with congestive HF and in those with atrial fibrillation, myocardial MR expression is increased.20,21 Renal MR expression is increased in animal disease models, such as streptozotocin-induced type 1 diabetes mellitus and type 2 diabetes mellitus.22 In patients with renal failure, MR expression is increased 5-fold in those with high albuminuria compared with patients with no or moderate albuminuria.23

Direct deleterious effects of aldosterone in the heart include development of myocardial hypertrophy, ventricular remodeling, proarrhythmogenic effects, myocardial ischemia, reduced coronary blood flow, and myocardial injury; the effects of aldosterone on the kidneys include glomerular hypertrophy, glomerulosclerosis, proteinuria, reduced renal blood flow, and renal injury (Figure).2428 Importantly, the aldosterone-induced damage to the heart and kidneys is largely independent of the systemic effects on blood pressure.10,29

Figure.

Figure. The direct deleterious effects of aldosterone/MR activation in the heart and kidneys and the common pathophysiological mechanisms involved. The benefits of MRAs in interrupting these pathways are also illustrated. 11-BHSD2 indicates 11β-hydroxysteroid dehydrogenase type 2; AP-1, activator protein-1; CTGF, connective tissue growth factor; NADPH oxidase, nicotinamide adenine dinucleotide phosphate-oxidase; MAPK, mitogen-activated protein kinase; MR, mineralocorticoid receptor; MRA, mineralocorticoid receptor antagonist; NF-κB, nuclear factor-κB; NO, nitric oxide; ROS, reactive oxygen species; SGK-1, serum- and glucocorticoid-induced protein kinase-1; and TGF-β, transforming growth factor-β.

Inflammation and fibrosis seem to play central roles in the pathophysiology of both cardiac and renal aldosterone-induced injury. In response to treatment with aldosterone and salt, rat myocardium displays increased inflammation and upregulated expression of proinflammatory cytokines, such as tumor necrosis factor-α, interleukin-1β, and transforming growth factor-β1 (TGF-β).25,30 In the rat kidney, aldosterone increases the expression of intercellular adhesion molecule-1.31 These effects may be mediated by serum- and glucocorticoid-induced protein kinase-1 and transcription factors nuclear factor-κB and activator protein-1, the expression and activity of which, respectively, are stimulated by aldosterone/salt or angiotensin II/salt in animal heart and kidneys.2933 Kidney biopsies from patients with high albuminuria show significant increases in the expression of serum- and glucocorticoid-induced protein kinase-1 and the inflammatory mediators macrophage chemoattractant protein-1, TGF-β, and interleukin-6 compared with those from patients with no or moderate albuminuria.23 Administration of aldosterone/salt in rats increases myocardial collagen synthesis and content, fibrosis, and profibrotic factors, including connective tissue growth factor, TGF-β, plasminogen activator inhibitor-1, matrix metalloproteinase-2, and tumor necrosis factor-α.30,3436 Renal fibrosis occurs in hypertensive transgenic rats with elevated serum aldosterone and proteinuria, and aldosterone is known to increase the renal expression of the fibrotic proteins osteopontin, fibrinogen, collagen type 1, plasminogen activator inhibitor-1, and connective tissue growth factor.26,31,37

Oxidative stress is a well-recognized trigger for inflammation and contributes to the development of fibrosis. In several animal models, increases in angiotensin II and aldosterone enhance oxidative stress in the heart, as is evident from increased nicotinamide adenine dinucleotide phosphate oxidase activity, expression of nicotinamide adenine dinucleotide phosphate oxidase components, and reactive oxygen species formation.30,34,35 Aldosterone-induced oxidative and nitrosative stress has also been demonstrated in the rat kidney.29 Increased oxidative stress, inflammation, and fibrosis are evident in several animal models of cardiac and renal diseases (eg, rats post-MI, dogs with HF, and uninephrectomized diabetic rats).18,3840

Furthermore, MR activation stimulates apoptosis and causes vasoconstriction and reduced blood flow in the animal heart and kidneys.8,18,41,42 Reduced endothelium-dependent vasodilatation is thought to be caused by an aldosterone-mediated decrease in the bioavailability of nitric oxide.

The requirement for a high-salt environment for aldosterone-mediated injury is common to both the cardiac and renal effects and was first observed almost 40 years ago.43 A high-salt diet may stimulate oxidative stress, which then promotes activation of the MR.44 Thus, there may be a synergism between activation of the MR and oxidative stress, although the mechanisms for this are yet to be fully elucidated.5 Cross-talk and interactions between the MR and the angiotensin receptor 1 have also been proposed to contribute to end-organ damage in both the heart and kidneys.45

Although the data from expression studies and investigations into the effects of aldosterone infusions have provided valuable insights into MR signaling, perhaps the most compelling evidence for the pathophysiological role of MR activation has come from the demonstration of the protective effects of the currently available MRAs, spironolactone, and eplerenone, in models of cardiac and renal diseases (Figure; also Table S1 in the online-only Data Supplement). Treatment with an MRA attenuates or prevents cardiac hypertrophy and the development of HF in several experimental models of heart disease (Table S1). MRAs also improve glomerulosclerosis, attenuate proteinuria, and reduce glomerular hypertrophy and renal injury in multiple animal models of kidney disease (Table S1).

MRAs suppress overactivation of the MR in rat models of hypertension and post-MI and in uninephrectomized diabetic rats (Table S1). MRA treatment in animal heart and kidneys, and in animal models of cardiac and renal disease, prevents or reduces inflammation, fibrosis, and expression of markers of oxidative stress induced by aldosterone/salt (Figure; Table S1). Treatment with spironolactone significantly attenuates the increases in cardiac expression of proinflammatory and profibrotic mediators, such as serum- and glucocorticoid-induced protein kinase-1, TGF-β, connective tissue growth factor, matrix metalloproteinase-2, interleukin-1β, and nicotinamide adenine dinucleotide phosphate oxidase components that are induced with aldosterone/salt.30 Reductions in renal expression of proinflammatory and profibrotic mediators, including TGF-β, connective tissue growth factor, and cytokines, have also been demonstrated with eplerenone in spontaneously hypertensive rats.46

In addition to these effects on inflammation and fibrosis, MRA treatment attenuates apoptosis and endothelial dysfunction and improves coronary and renal blood flow in animal models of cardiac and renal disease (Table S1). Improvements in endothelial function and blood flow with MRAs are associated with increases in endothelial nitric oxide synthase in both rat kidneys and hearts.47,48 Further benefits of MRAs include inhibition of electric remodeling and prevention of arrhythmogenic activity caused by aldosterone or disease states in mice, rat, and dog models and attenuation of podocyte injury in rat models of hypertension and diabetes mellitus (Table S1).

The beneficial effects of MRAs in preventing or attenuating cardiac and renal diseases in animal models are largely independent of the systemic hemodynamic changes, suggesting they are a result of blocking the direct deleterious effects of MR activation in the heart and kidneys.3,5,10

Clinical Evidence: MRAs in Cardiac and Renal Diseases

Cardiac Disease

In 3 landmark randomized controlled trials, MRA treatment in patients with HF and reduced left ventricular ejection fraction (LVEF) significantly reduced mortality and morbidity (Table S2). In the Randomized Aldactone Evaluation Study (RALES), spironolactone significantly reduced death from all causes, the frequency of hospitalization for worsening HF, and the severity of HF symptoms compared with placebo in patients with severe HF receiving standard therapy (Table S2). The reduction in mortality with spironolactone was attributed to a lower risk of death from progressive HF and of sudden death from cardiac causes. The Eplerenone Post-Myocardial Heart Failure Efficacy and Survival Study (EPHESUS) demonstrated significant reductions in death from any cause and in the rate of deaths or hospitalization from cardiovascular causes with eplerenone versus placebo in patients with HF and reduced LVEF post-MI (Table S2). More recently, the Eplerenone in Mild Patients Hospitalization and Survival Study in Heart Failure (EMPHASIS-HF) reported significant benefits of eplerenone on mortality and cardiovascular morbidity compared with placebo in patients with mild HF and reduced LVEF already receiving recommended therapy (Table S2).

Hyperkalemia is a significant adverse event and one of the most common concerns associated with the use of MRAs. The reported incidence of serious hyperkalemia in the landmark MRA HF trials was between 2% and 11.8%.4951

Subanalyses of the HF trials have provided support for preclinical data on the role of aldosterone/MR activation in the pathophysiology of cardiac disease. In RALES, spironolactone decreased from baseline serum levels of procollagen type 1 carboxy-terminal peptide, procollagen type 1 aminoterminal propeptide, and procollagen type III aminoterminal peptide, all of which are markers of cardiac fibrosis (Table S2). Similarly, in a subgroup analysis of EPHESUS, levels of collagen biomarkers were significantly lower in the eplerenone group than in the placebo group (Table S2).

The safety and tolerability of a novel nonsteroidal MRA, finerenone, were investigated in patients with HF with reduced LVEF and mild or moderate chronic kidney disease (CKD) in the phase 2 Mineralcocorticoid Receptor Antagonist Tolerability Study (ARTS; Table S2). Finerenone was at least as effective as spironolactone in decreasing levels of serum brain natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP), which are biomarkers of hemodynamic stress, and was associated with a lower incidence of hyperkalemia and worsening renal function.52 There was a significant decrease in blood pressure from baseline in patients receiving spironolactone, but not in those receiving finerenone or placebo.

Additional small-scale clinical studies have reported the benefits of MRA treatment on left ventricular modeling, diastolic function, and New York Heart Association class in patients with HF and reduced LVEF.4

The efficacy of MRAs in the treatment of patients with HF and preserved LVEF has also been investigated. The Aldosterone Receptor Blockade in Diastolic Heart Failure trial reported significant improvements in diastolic function and significant reductions in left ventricular mass index with spironolactone compared with placebo in patients with chronic HF, preserved LVEF, and echocardiographic evidence of diastolic dysfunction, although there were no significant differences in maximal exercise capacity, HF symptoms, or quality of life between the 2 groups (Table S2). The Treatment of Preserved Cardiac Function Heart Failure With an Aldosterone Antagonist (TOPCAT) trial investigated the efficacy of spironolactone in reducing mortality and morbidity in patients with HF and LVEF of ≥45% (Table S2). Spironolactone did not significantly reduce the number of patients who reached the primary composite end point of cardiovascular death, hospitalization for HF, or aborted cardiac arrest (nonsignificant reduction of 11%), but did significantly reduce the risk of hospitalization for HF compared with placebo.

The proven benefits of the addition of an MRA to standard therapy on long-term mortality and morbidity in patients with HF and a reduced LVEF is in contrast to the lack of clear evidence for the protective effects of adding an angiotensin receptor blocker (ARB) to angiotensin-converting enzyme (ACE) inhibitor therapy on such outcomes in these patients.53

There is also evidence of the benefits of MRAs in patients with other types of cardiac disease (Table S2). Eplerenone significantly reduced left ventricular mass and lowered systolic and diastolic blood pressure from baseline in patients with left ventricular hypertrophy and hypertension (Table S2). In patients with resistant hypertension, the addition of an MRA to standard therapy afforded significant reductions in blood pressure and left ventricular mass, which were independent of plasma aldosterone levels (Table S2). In patients with a first anterior MI, addition of spironolactone significantly improved LVEF, suppressed left ventricular end-diastolic volume index, and reduced markers of fibrosis compared with ACE inhibitor therapy alone (Table S2). In the recent Role of Eplerenone in Acute Myocardial Infarction–Double-Blind, Early Treatment Initiation, Randomized, placebo-controlled, multi-center study (REMINDER), eplerenone provided a significant reduction in the composite end point of cardiovascular mortality and morbidity and elevated BNP/NT-proBNP levels versus placebo in patients with acute ST-elevation MI and no history of HF (Table S2).

Renal Disease

Numerous small-scale clinical studies have demonstrated reductions in proteinuria or albuminuria with the addition of MRAs to ACE inhibitor/ARB therapy in patients with CKD and diabetic nephropathy (Table S3). In the majority of studies, these beneficial effects were independent of changes in blood pressure. An analysis of 8 studies reported a 23% to 61% reduction in albuminuria in patients with diabetic nephropathy with the addition of an MRA compared with standard treatment alone.54 Not unexpectedly, MRAs were associated with increases in mean serum potassium and incident hyperkalemia. In a prospective randomized open-label study in patients with CKD already receiving ACE inhibitors or ARBs, spironolactone slowed the decrease in estimated glomerular filtration rate, and therefore the progression of CKD, in addition to reducing proteinuria, compared with controls (Table S3). In patients with nondiabetic nephropathy, the addition of spironolactone reduced markers of tubular injury and fibrosis compared with ACE inhibitor and ARB therapy alone, supporting the preclinical findings of the effects of aldosterone/MR activation on the pathophysiology of renal disease (Table S3).

In ARTS, which assessed the effects of finerenone in patients with HF with reduced LVEF and mild or moderate CKD, the novel MRA was associated with similar reductions in the urinary albumin-to-creatinine ratio from baseline, significantly smaller decreases in estimated glomerular filtration rate from baseline and significantly lower incidences of worsening renal function, renal impairment, and renal dysfunction compared with spironolactone (Table S3). Although the mean increase in serum potassium concentration from baseline was significantly greater with some doses of finerenone than with placebo, these increases were significantly smaller than those in the spironolactone group.52

The efficacy and safety of MRAs in the treatment of patients with end-stage renal disease are now being explored (Table S3). Safety data on the use of MRAs in patients with end-stage renal disease undergoing hemodialysis suggest that the risk of hyperkalemia with low-dose MRAs is significantly lower than previously thought, and MRA treatment with close laboratory monitoring is a favorable treatment option in these patients.2 A recent randomized controlled trial in patients with oliguria undergoing hemodialysis demonstrated substantial reductions in cardiovascular and cerebrovascular mortality and morbidity with spironolactone compared with controls, providing a strong rationale for further investigations in large-scale clinical trials (Table S3).

Clinical Implications

The effectiveness of MRAs in some patients with cardiac disease, particularly HF, has been proven in clinical trials. Future challenges will be to assess the mortality and morbidity benefits of MRAs in a wider population of patients with cardiac disease, including high-risk patients with concomitant renal disease. Initial clinical findings suggest the potential efficacy of MRAs in the treatment of patients with CKD and diabetic nephropathy, but robust safety data, data on morbidity and mortality, and data on hard clinical outcomes assessing disease progression, such as loss of renal function and progression to end-stage renal disease, are still lacking and remain important goals for future clinical trials.

Hyperkalemia is a significant concern with MRAs and has limited the use of these agents in some populations with HF. It has also dampened enthusiasm for the development of MRAs as treatments for renal disease because hyperkalemia is a common complication of CKD.55 A higher incidence of hyperkalemia has been documented with MRAs in clinical practice than in the landmark HF clinical trials (incidences of 2%−11.8%), and increases in hyperkalemia-associated hospitalizations and deaths have been reported since publication of the first trial, RALES.56 Other studies, however, have described similar rates of hyperkalemia to those reported in the 3 landmark clinical trials and no significant increases in hyperkalemia-related hospitalizations and deaths in clinical practice following the RALES study.57 Indeed, the clinical relevance of reported increases in the risk of hyperkalemia warrants close examination. In RALES, the median potassium concentration in the spironolactone group was only 0.3 mmol/L greater than in the placebo group.51 In addition, it is important to note that in all 3 landmark HF trials, the overall beneficial effects of the MRAs on mortality and morbidity were consistent, regardless of serum potassium levels and worsening renal function, and were independent of the diuretic effects of the MRAs.5861 Furthermore, although hyperkalemia is the most common concern, the risk of hypokalemia also merits consideration. There is some evidence of an association between hypokalemia and increased mortality and morbidity, even in patients with CKD.62 In both EPHESUS and EMPHASIS-HF, the incidence of serious hypokalemia was lower in the MRA group than in the placebo group.49,50 Thus, when assessing the risks associated with MRAs, all of the effects on potassium levels and associated implications should be examined, particularly among patients with HF. The effects of MRAs on potassium metabolism have been proposed to underlie some of the benefits demonstrated in HF trials.63 Subanalyses of the landmark MRA trials in patients with HF, however, suggest that the benefits of MRA treatment are largely independent of their effects on potassium levels, and hyperkalemia remains the primary concern limiting the use of MRAs in patients with HF/CKD in clinical practice.5861 An increase in serum potassium levels can also occur during treatment with ACE inhibitors and ARBs and, by stimulating aldosterone synthesis and release from the adrenal glands, may contribute to aldosterone breakthrough, a phenomenon well-documented with chronic use of these therapies.64 The risk of hyperkalemia may be minimized by routine monitoring of serum potassium and renal function and avoidance of concomitant drugs and foods associated with potassium retention. A novel potassium-binding polymer, RLY5016, prevented hyperkalemia in patients with chronic HF receiving spironolactone and may have a role in preventing this complication, although further studies are required, particularly in patients with CKD.65 Additional preventive oral therapies are also under investigation.66 Novel MRAs with a more favorable safety profile than those of spironolactone and eplerenone may also significantly reduce the risk of hyperkalemia.

Additional drawbacks of available MRAs, most notably the antiandrogenic effects associated with spironolactone, have also contributed to underuse in clinical practice and the drive to discover the next generation of MRAs. The novel, nonsteroidal MRA finerenone has shown greater selectivity than spironolactone and greater potency for the MR than eplerenone (Table S4).67 Finerenone may also have greater selectivity for cardiovascular tissue than for renal tissue and, therefore, a potentially improved cardiac-to-renal activity ratio compared with available MRAs.67 The findings from ARTS that finerenone is at least as effective as spironolactone in reducing biomarkers of hemodynamic stress and is associated with lower incidences of hyperkalemia and renal adverse events than spironolactone in patients with HF, reduced LVEF, and moderate CKD provide support for this more favorable balance of cardiac and renal effects.52,68

Conclusions

Direct deleterious effects of aldosterone and MR activation, via mechanisms involving oxidative stress, inflammation, and fibrosis, occur in both the heart and kidneys. Clinical data support the findings of experimental studies and have shown that MRAs block these mechanisms and subsequent cardiac and renal injury independently of systemic hemodynamic changes. Such effects of MRAs are likely to play a major role in the proven benefits of these agents on mortality, morbidity, and disease progression in multiple types of cardiac disease and in the initial findings of similar benefits in renal disease. Ongoing and future clinical trials are merited to explore the potential of MRAs further in more patient populations with cardiac and renal disease. Hyperkalemia remains a concern with MRAs but may be addressed, in part, by novel MRAs, such as finerenone, which have shown a potentially more favorable cardiac-to-renal activity ratio.

Acknowledgments

J. Bauersachs and F. Jaisser get support from the FP7-funded COST ADMIRE network (BM1301). Editorial support was provided by Dr Charlotte Cookson and Dr Emma Moorhouse of Oxford PharmaGenesis™ Ltd.

Footnotes

The online-only Data Supplement is available with this article at http://hyper.ahajournals.org/lookup/suppl/doi:10.1161/HYPERTENSIONAHA.114.04488/-/DC1.

Correspondence to Johann Bauersachs, Department of Cardiology and Angiology, Centre for Internal Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hanover, Germany. E-mail

References

  • 1. Ronco C, Di Lullo L. Cardiorenal syndrome.Heart Fail Clin. 2014; 10:251–280.CrossrefMedlineGoogle Scholar
  • 2. Shavit L, Lifschitz MD, Epstein M. Aldosterone blockade and the mineralocorticoid receptor in the management of chronic kidney disease: current concepts and emerging treatment paradigms.Kidney Int. 2012; 81:955–968.CrossrefMedlineGoogle Scholar
  • 3. Bertocchio JP, Warnock DG, Jaisser F. Mineralocorticoid receptor activation and blockade: an emerging paradigm in chronic kidney disease.Kidney Int. 2011; 79:1051–1060.CrossrefMedlineGoogle Scholar
  • 4. Vizzardi E, Regazzoni V, Caretta G, Gavazzoni M, Sciatti E, Bonadei I, Trichaki E, Raddino R, Metra M. Mineralocorticoid receptor antagonists in heart failure: past, present and future perspectives.IJC Heart Vessels. 2014; 3:6–14.CrossrefGoogle Scholar
  • 5. Messaoudi S, Azibani F, Delcayre C, Jaisser F. Aldosterone, mineralocorticoid receptor, and heart failure.Mol Cell Endocrinol. 2012; 350:266–272.CrossrefMedlineGoogle Scholar
  • 6. Hostetter TH, Ibrahim HN. Aldosterone in chronic kidney and cardiac disease.J Am Soc Nephrol. 2003; 14:2395–2401.CrossrefMedlineGoogle Scholar
  • 7. Ferrari P. The role of 11β-hydroxysteroid dehydrogenase type 2 in human hypertension.Biochim Biophys Acta. 2010; 1802:1178–1187.CrossrefMedlineGoogle Scholar
  • 8. Benard L, Milliez P, Ambroisine ML, Messaoudi S, Samuel JL, Delcayre C. Effects of aldosterone on coronary function.Pharmacol Rep. 2009; 61:58–66.CrossrefMedlineGoogle Scholar
  • 9. Messaoudi S, Jaisser F. Aldosterone and the mineralocorticoid receptor.Eur Heart J Suppl. 2011; 13 (Suppl B):B4–B9.CrossrefGoogle Scholar
  • 10. Rentoukas EI, Lazaros GA, Zirogiannis PN. Aldosterone in heart and kidney diseases.Hellenic J Cardiol. 2005; 46:408–419.MedlineGoogle Scholar
  • 11. Krug AW, Pojoga LH, Williams GH, Adler GK. Cell membrane-associated mineralocorticoid receptors? New evidence.Hypertension. 2011; 57:1019–1025.LinkGoogle Scholar
  • 12. Falkenstein E, Christ M, Feuring M, Wehling M. Specific nongenomic actions of aldosterone.Kidney Int. 2000; 57:1390–1394.CrossrefMedlineGoogle Scholar
  • 13. Jaisser F, Swynghedauw B, Delcayre C. The mineralocorticoid receptor in heart: different effects in different cells.Hypertension. 2011; 57:679–680.LinkGoogle Scholar
  • 14. Lother A, Berger S, Gilsbach R, Rösner S, Ecke A, Barreto F, Bauersachs J, Schütz G, Hein L. Ablation of mineralocorticoid receptors in myocytes but not in fibroblasts preserves cardiac function.Hypertension. 2011; 57:746–754.LinkGoogle Scholar
  • 15. Fraccarollo D, Berger S, Galuppo P, Kneitz S, Hein L, Schütz G, Frantz S, Ertl G, Bauersachs J. Deletion of cardiomyocyte mineralocorticoid receptor ameliorates adverse remodeling after myocardial infarction.Circulation. 2011; 123:400–408.LinkGoogle Scholar
  • 16. de Resende MM, Kauser K, Mill JG. Regulation of cardiac and renal mineralocorticoid receptor expression by captopril following myocardial infarction in rats.Life Sci. 2006; 78:3066–3073.CrossrefMedlineGoogle Scholar
  • 17. Ouvrard-Pascaud A, Sainte-Marie Y, Bénitah JP, et al. Conditional mineralocorticoid receptor expression in the heart leads to life-threatening arrhythmias.Circulation. 2005; 111:3025–3033.LinkGoogle Scholar
  • 18. Takeda M, Tatsumi T, Matsunaga S, Hayashi H, Kimata M, Honsho S, Nishikawa S, Mano A, Shiraishi J, Yamada H, Takahashi T, Matoba S, Kobara M, Matsubara H. Spironolactone modulates expressions of cardiac mineralocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 2 and prevents ventricular remodeling in post-infarct rat hearts.Hypertens Res. 2007; 30:427–437.CrossrefMedlineGoogle Scholar
  • 19. Nagata K, Obata K, Xu J, Ichihara S, Noda A, Kimata H, Kato T, Izawa H, Murohara T, Yokota M. Mineralocorticoid receptor antagonism attenuates cardiac hypertrophy and failure in low-aldosterone hypertensive rats.Hypertension. 2006; 47:656–664.LinkGoogle Scholar
  • 20. Lavall D, Selzer C, Schuster P, Lenski M, Adam O, Schäfers HJ, Böhm M, Laufs U. The mineralocorticoid receptor promotes fibrotic remodeling in atrial fibrillation.J Biol Chem. 2014; 289:6656–6668.CrossrefMedlineGoogle Scholar
  • 21. Yoshida M, Ma J, Tomita T, Morikawa N, Tanaka N, Masamura K, Kawai Y, Miyamori I. Mineralocorticoid receptor is overexpressed in cardiomyocytes of patients with congestive heart failure.Congest Heart Fail. 2005; 11:12–16.CrossrefMedlineGoogle Scholar
  • 22. Guo C, Martinez-Vasquez D, Mendez GP, Toniolo MF, Yao TM, Oestreicher EM, Kikuchi T, Lapointe N, Pojoga L, Williams GH, Ricchiuti V, Adler GK. Mineralocorticoid receptor antagonist reduces renal injury in rodent models of types 1 and 2 diabetes mellitus.Endocrinology. 2006; 147:5363–5373.CrossrefMedlineGoogle Scholar
  • 23. Quinkler M, Zehnder D, Eardley KS, Lepenies J, Howie AJ, Hughes SV, Cockwell P, Hewison M, Stewart PM. Increased expression of mineralocorticoid effector mechanisms in kidney biopsies of patients with heavy proteinuria.Circulation. 2005; 112:1435–1443.LinkGoogle Scholar
  • 24. Rocha R, Rudolph AE, Frierdich GE, Nachowiak DA, Kekec BK, Blomme EA, McMahon EG, Delyani JA. Aldosterone induces a vascular inflammatory phenotype in the rat heart.Am J Physiol Heart Circ Physiol. 2002; 283:H1802–H1810.CrossrefMedlineGoogle Scholar
  • 25. López-Andrés N, Martin-Fernandez B, Rossignol P, Zannad F, Lahera V, Fortuno MA, Cachofeiro V, Díez J. A role for cardiotrophin-1 in myocardial remodeling induced by aldosterone.Am J Physiol Heart Circ Physiol. 2011; 301:H2372–H2382.CrossrefMedlineGoogle Scholar
  • 26. Fukuda S, Horimai C, Harada K, Wakamatsu T, Fukasawa H, Muto S, Itai A, Hayashi M. Aldosterone-induced kidney injury is mediated by NFκB activation.Clin Exp Nephrol. 2011; 15:41–49.CrossrefMedlineGoogle Scholar
  • 27. Dartsch T, Fischer R, Gapelyuk A, Weiergraeber M, Ladage D, Schneider T, Schirdewan A, Reuter H, Mueller-Ehmsen J, Zobel C. Aldosterone induces electrical remodeling independent of hypertension.Int J Cardiol. 2013; 164:170–178.CrossrefMedlineGoogle Scholar
  • 28. Greene EL, Kren S, Hostetter TH. Role of aldosterone in the remnant kidney model in the rat.J Clin Invest. 1996; 98:1063–1068.CrossrefMedlineGoogle Scholar
  • 29. Queisser N, Amann K, Hey V, Habib SL, Schupp N. Blood pressure has only minor influence on aldosterone-induced oxidative stress and DNA damage in vivo.Free Radic Biol Med. 2013; 54:17–25.CrossrefMedlineGoogle Scholar
  • 30. Martín-Fernández B, de las Heras N, Miana M, Ballesteros S, Delgado C, Song S, Hintze T, Cachofeiro V, Lahera V. Structural, functional, and molecular alterations produced by aldosterone plus salt in rat heart: association with enhanced serum and glucocorticoid-regulated kinase-1 expression.J Cardiovasc Pharmacol. 2011; 57:114–121.CrossrefMedlineGoogle Scholar
  • 31. Terada Y, Ueda S, Hamada K, Shimamura Y, Ogata K, Inoue K, Taniguchi Y, Kagawa T, Horino T, Takao T. Aldosterone stimulates nuclear factor-kappa B activity and transcription of intercellular adhesion molecule-1 and connective tissue growth factor in rat mesangial cells via serum- and glucocorticoid-inducible protein kinase-1.Clin Exp Nephrol. 2012; 16:81–88.CrossrefMedlineGoogle Scholar
  • 32. Neves MF, Amiri F, Virdis A, Diep QN, Schiffrin EL; CIHR Multidisciplinary Research Group on Hypertension. Role of aldosterone in angiotensin II-induced cardiac and aortic inflammation, fibrosis, and hypertrophy.Can J Physiol Pharmacol. 2005; 83:999–1006.CrossrefMedlineGoogle Scholar
  • 33. Han JS, Choi BS, Yang CW, Kim YS. Aldosterone-induced TGF-beta1 expression is regulated by mitogen-activated protein kinases and activator protein-1 in mesangial cells.J Korean Med Sci. 2009; 24Suppl:S195–S203.CrossrefMedlineGoogle Scholar
  • 34. Habibi J, DeMarco VG, Ma L, Pulakat L, Rainey WE, Whaley-Connell AT, Sowers JR. Mineralocorticoid receptor blockade improves diastolic function independent of blood pressure reduction in a transgenic model of RAAS overexpression.Am J Physiol Heart Circ Physiol. 2011; 300:H1484–H1491.CrossrefMedlineGoogle Scholar
  • 35. Kagiyama S, Matsumura K, Goto K, Otsubo T, Iida M. Role of Rho kinase and oxidative stress in cardiac fibrosis induced by aldosterone and salt in angiotensin type 1a receptor knockout mice.Regul Pept. 2010; 160:133–139.CrossrefMedlineGoogle Scholar
  • 36. Chun TY, Pratt JH. Aldosterone increases plasminogen activator inhibitor-1 synthesis in rat cardiomyocytes.Mol Cell Endocrinol. 2005; 239:55–61.CrossrefMedlineGoogle Scholar
  • 37. Whaley-Connell AT, Habibi J, Nistala R, DeMarco VG, Pulakat L, Hayden MR, Joginpally T, Ferrario CM, Parrish AR, Sowers JR. Mineralocorticoid receptor-dependent proximal tubule injury is mediated by a redox-sensitive mTOR/S6K1 pathway.Am J Nephrol. 2012; 35:90–100.CrossrefMedlineGoogle Scholar
  • 38. Taira M, Toba H, Murakami M, Iga I, Serizawa R, Murata S, Kobara M, Nakata T. Spironolactone exhibits direct renoprotective effects and inhibits renal renin-angiotensin-aldosterone system in diabetic rats.Eur J Pharmacol. 2008; 589:264–271.CrossrefMedlineGoogle Scholar
  • 39. Noda K, Kobara M, Hamada J, Yoshifuji Y, Shiraishi T, Tanaka T, Wang J, Toba H, Nakata T. Additive amelioration of oxidative stress and cardiac function by combined mineralocorticoid and angiotensin receptor blockers in postinfarct failing hearts.J Cardiovasc Pharmacol. 2012; 60:140–149.CrossrefMedlineGoogle Scholar
  • 40. Tanaka-Esposito C, Varahan S, Jeyaraj D, Lu Y, Stambler BS. Eplerenone-mediated regression of electrical activation delays and myocardial fibrosis in heart failure.J Cardiovasc Electrophysiol. 2014; 25:537–544.CrossrefGoogle Scholar
  • 41. Zhu D, Yu H, He H, Ding J, Tang J, Cao D, Hao L. Spironolactone inhibits apoptosis in rat mesangial cells under hyperglycaemic conditions via the Wnt signalling pathway.Mol Cell Biochem. 2013; 380:185–193.CrossrefMedlineGoogle Scholar
  • 42. Bobadilla NA, Gamba G. New insights into the pathophysiology of cyclosporine nephrotoxicity: a role of aldosterone.Am J Physiol Renal Physiol. 2007; 293:F2–F9.CrossrefMedlineGoogle Scholar
  • 43. Oliver WJ, Cohen EL, Neel JV. Blood pressure, sodium intake, and sodium related hormones in the Yanomamo Indians, a “no-salt” culture.Circulation. 1975; 52:146–151.LinkGoogle Scholar
  • 44. Kitada K, Nakano D, Liu Y, Fujisawa Y, Hitomi H, Shibayama Y, Shibata H, Nagai Y, Mori H, Masaki T, Kobori H, Nishiyama A. Oxidative stress-induced glomerular mineralocorticoid receptor activation limits the benefit of salt reduction in Dahl salt-sensitive rats.PLoS One. 2012; 7:e41896.CrossrefMedlineGoogle Scholar
  • 45. Jain G, Campbell RC, Warnock DG. Mineralocorticoid receptor blockers and chronic kidney disease.Clin J Am Soc Nephrol. 2009; 4:1685–1691.CrossrefMedlineGoogle Scholar
  • 46. Miana M, de Las Heras N, Rodriguez C, Sanz-Rosa D, Martin-Fernandez B, Mezzano S, Lahera V, Martinez-Gonzalez J, Cachofeiro V. Effect of eplerenone on hypertension-associated renal damage in rats: potential role of peroxisome proliferator activated receptor gamma (PPAR-γ).J Physiol Pharmacol. 2011; 62:87–94.MedlineGoogle Scholar
  • 47. Fraccarollo D, Galuppo P, Hildemann S, Christ M, Ertl G, Bauersachs J. Additive improvement of left ventricular remodeling and neurohormonal activation by aldosterone receptor blockade with eplerenone and ACE inhibition in rats with myocardial infarction.J Am Coll Cardiol. 2003; 42:1666–1673.CrossrefMedlineGoogle Scholar
  • 48. Kobayashi N, Hara K, Tojo A, Onozato ML, Honda T, Yoshida K, Mita S, Nakano S, Tsubokou Y, Matsuoka H. Eplerenone shows renoprotective effect by reducing LOX-1-mediated adhesion molecule, PKCepsilon-MAPK-p90RSK, and Rho-kinase pathway.Hypertension. 2005; 45:538–544.LinkGoogle Scholar
  • 49. Zannad F, McMurray JJ, Krum H, van Veldhuisen DJ, Swedberg K, Shi H, Vincent J, Pocock SJ, Pitt B; EMPHASIS-HF Study Group. Eplerenone in patients with systolic heart failure and mild symptoms.N Engl J Med. 2011; 364:11–21.CrossrefMedlineGoogle Scholar
  • 50. Pitt B, Remme W, Zannad F, Neaton J, Martinez F, Roniker B, Bittman R, Hurley S, Kleiman J, Gatlin M; Eplerenone Post-Acute Myocardial Infarction Heart Failure Efficacy and Survival Study Investigators. Eplerenone, a selective aldosterone blocker, in patients with left ventricular dysfunction after myocardial infarction.N Engl J Med. 2003; 348:1309–1321.CrossrefMedlineGoogle Scholar
  • 51. Pitt B, Zannad F, Remme WJ, Cody R, Castaigne A, Perez A, Palensky J, Wittes J. The effect of spironolactone on morbidity and mortality in patients with severe heart failure. Randomized Aldactone Evaluation Study Investigators.N Engl J Med. 1999; 341:709–717.CrossrefMedlineGoogle Scholar
  • 52. Pitt B, Kober L, Ponikowski P, Gheorghiade M, Filippatos G, Krum H, Nowack C, Kolkhof P, Kim SY, Zannad F. Safety and tolerability of the novel non-steroidal mineralocorticoid receptor antagonist BAY 94-8862 in patients with chronic heart failure and mild or moderate chronic kidney disease: a randomized, double-blind trial.Eur Heart J. 2013; 34:2453–2463.CrossrefMedlineGoogle Scholar
  • 53. McMurray JJ, Adamopoulos S, Anker SD, et al.; ESC Committee for Practice Guidelines. ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure 2012: the Task Force for the Diagnosis and Treatment of Acute and Chronic Heart Failure 2012 of the European Society of Cardiology. Developed in collaboration with the Heart Failure Association (HFA) of the ESC.Eur Heart J. 2012; 33:1787–1847.CrossrefMedlineGoogle Scholar
  • 54. Mavrakanas TA, Gariani K, Martin PY. Mineralocorticoid receptor blockade in addition to angiotensin converting enzyme inhibitor or angiotensin II receptor blocker treatment: an emerging paradigm in diabetic nephropathy: a systematic review.Eur J Intern Med. 2014; 25:173–176.CrossrefMedlineGoogle Scholar
  • 55. Miao Y, Dobre D, Heerspink HJ, Brenner BM, Cooper ME, Parving HH, Shahinfar S, Grobbee D, de Zeeuw D. Increased serum potassium affects renal outcomes: a post hoc analysis of the Reduction of Endpoints in NIDDM with the Angiotensin II Antagonist Losartan (RENAAL) trial.Diabetologia. 2011; 54:44–50.CrossrefMedlineGoogle Scholar
  • 56. Juurlink DN, Mamdani MM, Lee DS, Kopp A, Austin PC, Laupacis A, Redelmeier DA. Rates of hyperkalemia after publication of the Randomized Aldactone Evaluation Study.N Engl J Med. 2004; 351:543–551.CrossrefMedlineGoogle Scholar
  • 57. Wei L, Struthers AD, Fahey T, Watson AD, Macdonald TM. Spironolactone use and renal toxicity: population based longitudinal analysis.BMJ. 2010; 340:c1768.CrossrefMedlineGoogle Scholar
  • 58. Pitt B, Bakris G, Ruilope LM, DiCarlo L, Mukherjee R; EPHESUS Investigators. Serum potassium and clinical outcomes in the Eplerenone Post-Acute Myocardial Infarction Heart Failure Efficacy and Survival Study (EPHESUS).Circulation. 2008; 118:1643–1650.LinkGoogle Scholar
  • 59. Rossignol P, Ménard J, Fay R, Gustafsson F, Pitt B, Zannad F. Eplerenone survival benefits in heart failure patients post-myocardial infarction are independent from its diuretic and potassium-sparing effects. Insights from an EPHESUS (Eplerenone Post-Acute Myocardial Infarction Heart Failure Efficacy and Survival Study) substudy.J Am Coll Cardiol. 2011; 58:1958–1966.CrossrefMedlineGoogle Scholar
  • 60. Rossignol P, Dobre D, McMurray JJ, Swedberg K, Krum H, van Veldhuisen DJ, Shi H, Messig M, Vincent J, Girerd N, Bakris G, Pitt B, Zannad F. Incidence, determinants, and prognostic significance of hyperkalemia and worsening renal function in patients with heart failure receiving the mineralocorticoid receptor antagonist eplerenone or placebo in addition to optimal medical therapy: results from the Eplerenone in Mild Patients Hospitalization and Survival Study in Heart Failure (EMPHASIS-HF).Circ Heart Fail. 2014; 7:51–58.LinkGoogle Scholar
  • 61. Vardeny O, Claggett B, Anand I, Rossignol P, Desai AS, Zannad F, Pitt B, Solomon SD; Randomized Aldactone Evaluation Study (RALES) Investigators. Incidence, predictors, and outcomes related to hypo- and hyperkalemia in patients with severe heart failure treated with a mineralocorticoid receptor antagonist.Circ Heart Fail. 2014; 7:573–579.LinkGoogle Scholar
  • 62. Bowling CB, Pitt B, Ahmed MI, Aban IB, Sanders PW, Mujib M, Campbell RC, Love TE, Aronow WS, Allman RM, Bakris GL, Ahmed A. Hypokalemia and outcomes in patients with chronic heart failure and chronic kidney disease: findings from propensity-matched studies.Circ Heart Fail. 2010; 3:253–260.LinkGoogle Scholar
  • 63. Marciniak TA. Eplerenone treatment of aortic stenosis. [letter].Am Heart J. 2009; 157:e15; author reply e17.CrossrefMedlineGoogle Scholar
  • 64. Navaneethan SD, Bravo EL. Aldosterone breakthrough during angiotensin receptor blocker use: more questions than answers?Clin J Am Soc Nephrol. 2013; 8:1637–1639.CrossrefMedlineGoogle Scholar
  • 65. Pitt B, Anker SD, Bushinsky DA, Kitzman DW, Zannad F, Huang IZ; PEARL-HF Investigators. Evaluation of the efficacy and safety of RLY5016, a polymeric potassium binder, in a double-blind, placebo-controlled study in patients with chronic heart failure (the PEARL-HF) trial.Eur Heart J. 2011; 32:820–828.CrossrefMedlineGoogle Scholar
  • 66. McCullough PA, Beaver TM, Bennett-Guerrero E, Emmett M, Fonarow GC, Goyal A, Herzog CA, Kosiborod M, Palmer BF. Acute and chronic cardiovascular effects of hyperkalemia: new insights into prevention and clinical management.Rev Cardiovasc Med. 2014; 15:11–23.CrossrefMedlineGoogle Scholar
  • 67. Kolkhof P, Borden SA. Molecular pharmacology of the mineralocorticoid receptor: prospects for novel therapeutics.Mol Cell Endocrinol. 2012; 350:310–317.CrossrefMedlineGoogle Scholar
  • 68. Bauersachs J. The ARTS of third-generation mineralocorticoid receptor antagonists: achieving cardiovascular benefit with minimized renal side effects?Eur Heart J. 2013; 34:2426–2428.CrossrefMedlineGoogle Scholar

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